| Autor: |
Wenzlow N; Louisiana Animal Disease Diagnostic Laboratory, Louisiana State University, Baton Rouge, LA, USA., Mills D; Department of Biological Sciences and International Forensic Research Institute, Florida International University, Miami, FL, USA., Byrd J; Maples Center for Forensic Medicine, University of Florida, Gainesville, FL, USA., Warren M; Maples Center for Forensic Medicine, University of Florida, Gainesville, FL, USA., Long MT; Department of Comparative, Diagnostic, and Population Medicine, University of Florida, Gainesville, FL, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2023 Mar; Vol. 35 (2), pp. 97-108. Date of Electronic Publication: 2023 Feb 06. |
| DOI: |
10.1177/10406387231153930 |
| Abstrakt: |
We provide here an overview of the state of applied techniques in the estimation of the early period of the postmortem interval (PMI). The biological methods included consist of body cooling, CSF potassium, body cooling combined with CSF potassium, and tissue autolysis. For each method, we present its application in human and veterinary medicine and provide current methodology, strengths, and weaknesses, as well as target areas for improvement. We examine current and future molecular methods as they pertain to DNA and primarily to messenger RNA degradation for the estimation of the PMI, as well as the use of RNA in aging wounds, aging blood stains, and the identification of body fluids. Various types of RNA have different lengths, structures, and functions in cells. These differences in RNAs determine various intrinsic properties, such as their half-lives in cells, and, hence, their decay rate as well as their unique use for specific forensic tests. Future applications and refinements of RNA-based techniques provide opportunities for the use of molecular methods in the estimation of PMI and other general forensic applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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