LINE-1 retrotransposon expression in cancerous, epithelial and neuronal cells revealed by 5' single-cell RNA-Seq.

Autor:	McKerrow W; Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA., Kagermazova L; Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA., Doudican N; Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY, USA., Frazzette N; Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY, USA., Kaparos EI; Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA., Evans SA; Center for Computational Molecular Biology, Brown University, Providence, RI, USA.; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA., Rocha A; Center for Computational Molecular Biology, Brown University, Providence, RI, USA.; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA., Sedivy JM; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA.; Center on the Biology of Aging, Brown University, Providence, RI, USA., Neretti N; Center for Computational Molecular Biology, Brown University, Providence, RI, USA.; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI, USA., Carucci J; Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY, USA., Boeke JD; Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA.; Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn,NY11201, USA., Fenyö D; Institute for Systems Genetics, NYU Langone Health, New York, NY, USA.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York, NY, USA.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2023 Mar 21; Vol. 51 (5), pp. 2033-2045.
DOI:	10.1093/nar/gkad049
Abstrakt:	LINE-1 retrotransposons are sequences capable of copying themselves to new genomic loci via an RNA intermediate. New studies implicate LINE-1 in a range of diseases, especially in the context of aging, but without an accurate understanding of where and when LINE-1 is expressed, a full accounting of its role in health and disease is not possible. We therefore developed a method-5' scL1seq-that makes use of a widely available library preparation method (10x Genomics 5' single cell RNA-seq) to measure LINE-1 expression in tens of thousands of single cells. We recapitulated the known pattern of LINE-1 expression in tumors-present in cancer cells, absent from immune cells-and identified hitherto undescribed LINE-1 expression in human epithelial cells and mouse hippocampal neurons. In both cases, we saw a modest increase with age, supporting recent research connecting LINE-1 to age related diseases. (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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