Carbohydrate vitrification in aerosolized saliva is associated with the humidity-dependent infectious potential of airborne coronavirus.
Autor: | Nieto-Caballero M; Environmental Engineering Program, University of Colorado, Boulder, CO 80309, USA., Davis RD; Department of Chemistry, Trinity University, San Antonio, TX 78212, USA.; Materials Reliability Department, Sandia National Laboratories, Albuquerque, NM 82123, USA., Fuques E; Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA., Gomez OM; Environmental Engineering Program, University of Colorado, Boulder, CO 80309, USA., Huynh E; Department of Chemistry, Trinity University, San Antonio, TX 78212, USA., Handorean A; Departments of Engineering Design and Society and Civil and Environmental Engineering, Colorado School of Mines, Golden, CO 80401, USA., Ushijima S; Cooperative Institute for Research in Environmental Sciences and Department of Chemistry, University of Colorado, Boulder, CO 80309, USA., Tolbert M; Cooperative Institute for Research in Environmental Sciences and Department of Chemistry, University of Colorado, Boulder, CO 80309, USA., Hernandez M; Environmental Engineering Program, University of Colorado, Boulder, CO 80309, USA. |
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Jazyk: | angličtina |
Zdroj: | PNAS nexus [PNAS Nexus] 2022 Dec 24; Vol. 2 (2), pp. pgac301. Date of Electronic Publication: 2022 Dec 24 (Print Publication: 2023). |
DOI: | 10.1093/pnasnexus/pgac301 |
Abstrakt: | An accepted murine analogue for the environmental behavior of human SARS coronaviruses was aerosolized in microdroplets of its culture media and saliva to observe the decay of its airborne infectious potential under relative humidity (RH) conditions relevant to conditioned indoor air. Contained in a dark, 10 m 3 chamber maintained at 22°C, murine hepatitis virus (MHV) was entrained in artificial saliva particles that were aerosolized in size distributions that mimic SARS-CoV-2 virus expelled from infected humans' respiration. As judged by quantitative PCR, more than 95% of the airborne MHV aerosolized was recovered from microdroplets with mean aerodynamic diameters between 0.56 and 5.6 μm. As judged by its half-life, calculated from the median tissue culture infectious dose (TCID (© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.) |
Databáze: | MEDLINE |
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