In vivo imaging of axonal transport in peripheral nerves of rodent forelimbs.

Autor: Lang Q; Department of Neuromuscular Diseases and UCL Queen Square Motor Neuron Disease Centre, Queen Square Institute of Neurology, University College London, London WC1N 3BG, U.K., Schiavo G; Department of Neuromuscular Diseases and UCL Queen Square Motor Neuron Disease Centre, Queen Square Institute of Neurology, University College London, London WC1N 3BG, U.K.; UK Dementia Research Institute, University College London, London WC1E 6BT, U.K., Sleigh JN; Department of Neuromuscular Diseases and UCL Queen Square Motor Neuron Disease Centre, Queen Square Institute of Neurology, University College London, London WC1N 3BG, U.K.; UK Dementia Research Institute, University College London, London WC1E 6BT, U.K.
Jazyk: angličtina
Zdroj: Neuronal signaling [Neuronal Signal] 2023 Jan 19; Vol. 7 (1), pp. NS20220098. Date of Electronic Publication: 2023 Jan 19 (Print Publication: 2023).
DOI: 10.1042/NS20220098
Abstrakt: Axonal transport is the essential process by which neurons actively traffic a variety of cargoes between the cell soma and axon terminals. Accordingly, dysfunctional axonal transport is linked to many nervous system conditions. Therefore, being able to image and quantify this dynamic process in live neurons of animal disease models is beneficial for understanding neuropathology and testing new therapies at the preclinical level. As such, intravital approaches have been developed to assess cargo movement in the hindlimb sciatic nerves of live, anaesthetised mice. Here, we describe an adapted method for in vivo imaging of axonal transport in intact median and ulnar nerves of the rodent forelimb. Injection of a fluorescently labelled and non-toxic fragment of tetanus neurotoxin (H C T) into the mouse forepaw permits the identification of signalling endosomes in intact axons of median and ulnar nerves. Through immunofluorescent analysis of forelimb lumbrical muscles and median/ulnar nerves, we confirmed that H C T is taken up at motor nerve terminals and predominantly locates to motor axons. We then showed that the baseline trafficking of signalling endosomes is similar between the median/ulnar nerves and the sciatic nerve in adult wild-type mice. Importantly, this adapted method can be readily tailored for assessment of additional cargoes, such as mitochondria. By measuring transport in forelimb and hindlimb nerves, comparative anatomical and functional analyses can be performed in rodent disease models to aid our understanding of peripheral nerve disease pathogenesis and response to injury.
Competing Interests: The authors declare that there are no competing interests associated with the manuscript.
(© 2023 The Author(s).)
Databáze: MEDLINE
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