Planar carbon electrodes for real-time quantification of hydrogen sulfide release from cells.

Autor: Hall JR; Department of Chemistry, The University of North Carolina at Chapel Hill Chapel Hill North Carolina 27599 USA schoenfisch@unc.edu., Taylor JB; Department of Chemistry, The University of North Carolina at Chapel Hill Chapel Hill North Carolina 27599 USA schoenfisch@unc.edu., Bradshaw TM; Department of Chemistry, The University of North Carolina at Chapel Hill Chapel Hill North Carolina 27599 USA schoenfisch@unc.edu., Schoenfisch MH; Department of Chemistry, The University of North Carolina at Chapel Hill Chapel Hill North Carolina 27599 USA schoenfisch@unc.edu.; Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy Chapel Hill NC 27599 USA.
Jazyk: angličtina
Zdroj: Sensors & diagnostics [Sens Diagn] 2022 Dec 01; Vol. 2 (1), pp. 203-211. Date of Electronic Publication: 2022 Dec 01 (Print Publication: 2023).
DOI: 10.1039/d2sd00179a
Abstrakt: A planar electrode system was developed to permit the real-time, selective detection of hydrogen sulfide (H 2 S) from stimulated cells. Planar carbon electrodes were produced via stencil printing carbon ink through a laser cut vinyl mask. Electrodes were preconditioned using a constant potential amperometry methodology to prevent sensor drift resulting from elemental sulfur adsorption. Modification with a bilaminar coating (electropolymerized ortho -phenylenediamine and a fluorinated xerogel) facilitated high selectivity to H 2 S. To demonstrate the biological application of this planar sensor system, H 2 S released from 17β-estradiol-stimulated human umbilical vein endothelial cells (HUVECs) was quantified in situ in real-time. Stimulated HUVECs released sustained H 2 S levels for hours before returning to baseline. Cellular viability assays demonstrated negligible cell cytotoxicity at the electrochemical potentials required for analysis.
Competing Interests: None.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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