Investigating the photosensitization activities of flavins irradiated by blue LEDs.

Autor: Ribes J; University of Bordeaux, CNRS, CBMN, UMR 5248, Institute of Chemistry and Biology of Membranes & Nano-objects (CBMN) Allée Geoffroy Saint Hilaire, Bât B14 33600 Pessac France edouard.badarau@u-bordeaux.fr., Cossard P; University of Bordeaux, CNRS, CBMN, UMR 5248, Institute of Chemistry and Biology of Membranes & Nano-objects (CBMN) Allée Geoffroy Saint Hilaire, Bât B14 33600 Pessac France edouard.badarau@u-bordeaux.fr., Al Yaman K; University of Bordeaux, CNRS, CBMN, UMR 5248, Institute of Chemistry and Biology of Membranes & Nano-objects (CBMN) Allée Geoffroy Saint Hilaire, Bât B14 33600 Pessac France edouard.badarau@u-bordeaux.fr., Bestel I; University of Bordeaux, CNRS, CBMN, UMR 5248, Institute of Chemistry and Biology of Membranes & Nano-objects (CBMN) Allée Geoffroy Saint Hilaire, Bât B14 33600 Pessac France edouard.badarau@u-bordeaux.fr., Badarau E; University of Bordeaux, CNRS, CBMN, UMR 5248, Institute of Chemistry and Biology of Membranes & Nano-objects (CBMN) Allée Geoffroy Saint Hilaire, Bât B14 33600 Pessac France edouard.badarau@u-bordeaux.fr.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2023 Jan 16; Vol. 13 (4), pp. 2355-2364. Date of Electronic Publication: 2023 Jan 16 (Print Publication: 2023).
DOI: 10.1039/d2ra07379j
Abstrakt: Due to their ability to easily absorb light and to generate highly reactive species, photosensitizers emerged as promising tools in a wide variety of physico-chemical and biological processes. Natural photosensitizers have the benefit of a life-compatible toxicological profile. Porphyrins and flavins are such examples that already proved their efficiency as photo-dynamic therapeutics. The present article describes a reliable, easy-to-implement, readily available and reproducible method that can be used to characterize the photosensitizing activity of flavins. Several key factors were investigated during this study, the optimum parameters were: (i) a blue LED light source ( λ em = 455 nm) at 6.69 mW; (ii) a pH of 6 mimicking the tumoral environment; (iii) an air-saturated atmosphere reaction medium, (iv) a tetrazolium dye (MTT) was used to monitor the photosensitization efficacy via the generation of the colored MTT-formazan product. This method can be used to rank a series of flavins based on their photosensitizing activities. Such structure-photosensitization activity relationships are essential for the discovery of future potent photosensitizers for photodynamic therapy.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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