| Autor: |
El-Akhras BA; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, 392053Sadat City University, Egypt., Talaat RM; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, 392053Sadat City University, Egypt., El-Masry SA; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, 392053Sadat City University, Egypt., Bassyouni IH; Rheumatology and Rehabilitation Department, Faculty of Medicine, 63527Cairo University, Cairo, Egypt., El-Sayed IH; Biochemistry Department, Faculty of Science, 289154Kafr El-Sheikh University, Egypt., Ali YB; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, 392053Sadat City University, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of immunopathology and pharmacology [Int J Immunopathol Pharmacol] 2023 Jan-Dec; Vol. 37, pp. 3946320231154998. |
| DOI: |
10.1177/03946320231154998 |
| Abstrakt: |
microRNA-146a (miR-146a) plays an essential role in immune anomalies and organ injury of systemic lupus erythematosus (SLE) by regulating the disease's inflammation and complications. Here, we analyzed the expression of miR-146a in SLE and a panel of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-17, and TNF-α). Association between all measured parameters and the disease's clinical manifestation and response to treatment was monitored. Our study populations were 113 SLE patients and 104 healthy volunteers. miR-146a expression in peripheral blood mononuclear cells (PBMCs) was measured by quantitative real-time PCR (RT-qPCR). The content of the plasma cytokines (IL-1β, IL-6, IL-8, IL-17, and TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA). Compared with healthy controls, miR-146a expression was significantly increased ( p < 0.05) in lupus patients. The analysis of the receiver operator characteristic curve (ROC) of miR-146a showed 91% sensitivity and 70% specificity. IL-1β, IL-6, and IL-17 cytokines were significantly increased ( p < 0.001), while IL-8 and TNF-α were significantly decreased ( p < 0.001) in SLE patients against controls. The expression of miR-146a and TNF-α was upregulated considerably in SLE patients with severe disease activity. miR-146a expression was positively correlated with IL-6. Our results pointed to the elevation of miR-146a as a trade marker of SLE patients. Reduction of IL-8 and TNF-α in combination with an elevation of IL-1β, IL-6, and IL-17 might refer to miR-146a's dual effect in controlling inflammation in lupus. Although we shed some light on the role of miR-146a in SLE, further study is recommended to improve our results. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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