The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131.

Autor: Shahbazi R; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Deh Vank Ave., Tehran, 1993891176, Iran., Salmanzadeh-Ahrabi S; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Deh Vank Ave., Tehran, 1993891176, Iran. s.salmanzadeh@alzahra.ac.ir., Aslani MM; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Alebouyeh M; Pediatric Infections Research Center, Research for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Falahi J; Health Clinical Science Research Center, Zahedan Branch, Islamic Azad University, Zahedan, Iran., Nikbin VS; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Jazyk: angličtina
Zdroj: BMC pediatrics [BMC Pediatr] 2023 Feb 03; Vol. 23 (1), pp. 59. Date of Electronic Publication: 2023 Feb 03.
DOI: 10.1186/s12887-023-03866-w
Abstrakt: Background: Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered as multidrug-resistant and hypervirulent organism. There is lack of data about involvement of this pathogen in the children's infection. In this study, the prevalence, and clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 compared with non-O25-B2-ST131 isolates were investigated in children with urinary tract infection in Tehran, Iran.
Methods: The E. coli isolates from urine samples were identified using conventional microbiological methods. Characterization of E. coli O25-B2-ST131 clone, antibiotic susceptibility, biofilm formation, ESBLs phenotype and genotype, serum resistance, hemolysis, hydrophobicity, and formation of curli fimbriae were done using conventional microbiological and molecular methods. Clonality of the isolates was done by rep-PCR typing.
Results: Among 120 E. coli isolates, the highest and lowest antibiotic resistance was detected against ampicillin (92, 76.6%) and imipenem 5, (4.1%), respectively. Sixty-eight (56.6%) isolates were ESBL-producing and 58 (48.3%) isolates were considered as multi-drug resistance (MDR). The prevalence of ESBL-producing and MDR isolates in O25-B2-ST131 strains was higher compared with the non-O25-B2-ST131 strains (p value < 0.05). O25-B2-ST131 strains showed significant correlation with serum resistance and biofilm formation. Amongst the resistance and virulence genes, the prevalence of iucD, kpsMTII, cnf1, vat, bla CTX-M-15, and bla SHV were significantly higher among O25-B2-ST131 isolates in comparison with non-O25-B2-ST131 isolates (p value < 0.05). Considering a ≥ 80% homology cut-off, fifteen different clusters of the isolates were shown with the same rep-PCR pattern.
Conclusions: Our results confirmed the involvement of MDR-ESBLs producing E. coli strain O25-B2-ST131 in the occurrence of UTIs among children. Source tracking and control measures seem to be necessary for containment of the spread of hypervirulent and resistance variants in children.
(© 2023. The Author(s).)
Databáze: MEDLINE
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