Intrasexual cuticular hydrocarbon dimorphism in a wasp sheds light on hydrocarbon biosynthesis genes in Hymenoptera.

Autor: Moris VC; Department of Evolutionary Biology and Ecology, Institute of Biology I (Zoology), University of Freiburg, 79104, Freiburg, Germany. victoria.carla.moris@gmail.com.; Laboratory of Molecular Biology & Evolution (MBE), Department of Biology, Université Libre de Bruxelles, 1000, Brussels, Belgium. victoria.carla.moris@gmail.com., Podsiadlowski L; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany.; Institute of Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany., Martin S; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany.; Institute of Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany., Oeyen JP; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany.; Centre for Ecological and Evolutionary Synthesis, Department of Biosciences, University of Oslo, NO-0316, Oslo, Norway., Donath A; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany., Petersen M; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany.; High Performance Computing & Analytics Lab, University of Bonn, Friedrich-Hirzebruch-Allee 8, 53115, Bonn, Germany., Wilbrandt J; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany.; Leibniz Institute on Aging - Fritz Lipmann Institute, Beutenbergstraße 11, 07745, Jena, Germany., Misof B; Centre for Molecular Biodiversity Research, Leibniz Institute for the Analysis of Biodiversity Change / ZFMK, Museum Koenig, Adenauerallee 160, 53113, Bonn, Germany., Liedtke D; Institute of Human Genetics, University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Thamm M; Department of Behavioral Physiology and Sociobiology, University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Scheiner R; Department of Behavioral Physiology and Sociobiology, University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Schmitt T; Department of Animal Ecology and Tropical Biology Biocenter, University of Würzburg, Am Hubland, 97074, Würzburg, Germany., Niehuis O; Department of Evolutionary Biology and Ecology, Institute of Biology I (Zoology), University of Freiburg, 79104, Freiburg, Germany. oliver.niehuis@biologie.uni-freiburg.de.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Feb 03; Vol. 6 (1), pp. 147. Date of Electronic Publication: 2023 Feb 03.
DOI: 10.1038/s42003-022-04370-0
Abstrakt: Cuticular hydrocarbons (CHCs) cover the cuticle of insects and serve as desiccation barrier and as semiochemicals. While the main enzymatic steps of CHC biosynthesis are well understood, few of the underlying genes have been identified. Here we show how exploitation of intrasexual CHC dimorphism in a mason wasp, Odynerus spinipes, in combination with whole-genome sequencing and comparative transcriptomics facilitated identification of such genes. RNAi-mediated knockdown of twelve candidate gene orthologs in the honey bee, Apis mellifera, confirmed nine genes impacting CHC profile composition. Most of them have predicted functions consistent with current knowledge of CHC metabolism. However, we found first-time evidence for a fatty acid amide hydrolase also influencing CHC profile composition. In situ hybridization experiments furthermore suggest trophocytes participating in CHC biosynthesis. Our results set the base for experimental CHC profile manipulation in Hymenoptera and imply that the evolutionary origin of CHC biosynthesis predates the arthropods' colonization of land.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje