Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration.

Autor:	Cho E; Department of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea., Che X; Department of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, Skeletal Diseases Analysis Center, Korea Mouse Phenotyping Center (KMPC), School of Medicine, Kyungpook National University, Daegu, Republic of Korea., Ang MJ; Department of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Los Baños, Philippines., Cheon S; School of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea.; Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea., Lee J; Department of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea., Kim KS; Department of Microbiology, Department of Molecular Medicine (BK21plus), Chonnam National University Medical School, Gwangju, Republic of Korea., Lee CH; Therapeutic & Biotechnology Division, Drug Discovery Platform Research Center, Research Institute of Chemical Technology (KRICT), Daejeon, Republic of Korea., Lee SY; Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Ochang, Republic of Korea., Yang HY; Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea., Moon C; Department of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Republic of Korea., Park C; School of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea., Choi JY; Department of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, Skeletal Diseases Analysis Center, Korea Mouse Phenotyping Center (KMPC), School of Medicine, Kyungpook National University, Daegu, Republic of Korea., Lee TH; Department of Oral Biochemistry, Korea Mouse Phenotype Center (KMPC), Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Feb 03; Vol. 12. Date of Electronic Publication: 2023 Feb 03.
DOI:	10.7554/eLife.80122
Abstrakt:	Peroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function in the bone remains largely unknown. Here, we show that Prdx5 is involved in osteoclast and osteoblast differentiation, resulting in osteoporotic phenotypes in Prdx5 knockout ( Prdx5 Ko ) male mice. To investigate the function of Prdx5 in the bone, osteoblasts were analyzed through immunoprecipitation (IP) and liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) methods, while osteoclasts were analyzed through RNA-sequencing. Heterogeneous nuclear ribonucleoprotein K (hnRNPK) was identified as a potential binding partner of Prdx5 during osteoblast differentiation in vitro. Prdx5 acts as a negative regulator of hnRNPK-mediated osteocalcin ( Bglap ) expression. In addition, transcriptomic analysis revealed that in vitro differentiated osteoclasts from the bone marrow-derived macrophages of Prdx5 Ko mice showed enhanced expression of several osteoclast-related genes. These findings indicate that Prdx5 might contribute to the maintenance of bone homeostasis by regulating osteoblast differentiation. This study proposes a new function of Prdx5 in bone remodeling that may be used in developing therapeutic strategies for bone diseases. Competing Interests: EC, XC, MA, SC, JL, KK, CL, SL, HY, CM, CP, JC, TL No competing interests declared (© 2023, Cho, Che et al.)
Databáze:	MEDLINE
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