Whole-Cell MALDI-ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactions.

Autor: Aiosa N; School of Chemistry and Biochemistry, Georgia Institute of Technology, 950 Atlantic Drive, Atlanta, GA 30332, USA., Sinha A; Biotechnology & Bioengineering, Sandia National Laboratories, 7011 East Avenue, Livermore, CA 94550, USA., Albataineh H; School of Chemistry and Biochemistry, Georgia Institute of Technology, 950 Atlantic Drive, Atlanta, GA 30332, USA., Phillips AM; Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA., Mageeney CM; Biotechnology & Bioengineering, Sandia National Laboratories, 7011 East Avenue, Livermore, CA 94550, USA., Wilde DS; Department of Bioengineering, University of Pennsylvania, 210 South 33rd Street, Philadelphia, PA 19104, USA., Williams KP; Systems Biology, Sandia National Laboratories, 7011 East Avenue, Livermore, CA 94550, USA., Collette NM; Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA., Branda SS; Systems Biology, Sandia National Laboratories, 7011 East Avenue, Livermore, CA 94550, USA., Garg N; School of Chemistry and Biochemistry, Georgia Institute of Technology, 950 Atlantic Drive, Atlanta, GA 30332, USA.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, 311 Ferst Drive, ES&T, Atlanta, GA 30332, USA.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2023 Apr 03; Vol. 24 (7), pp. e202200802. Date of Electronic Publication: 2023 Mar 10.
DOI: 10.1002/cbic.202200802
Abstrakt: The emergence of drug-resistant pathogens necessitates the development of new countermeasures. In this regard, the introduction of probiotics to directly attack or competitively exclude pathogens presents a useful strategy. Application of this approach requires an understanding of how a probiotic and its target pathogen interact. A key means of probiotic-pathogen interaction involves the production of small molecules called natural products (NPs). Here, we report the use of whole-cell matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry to characterize NP production by candidate probiotics (mouse airway microbiome isolates) when co-cultured with the respiratory pathogen Burkholderia. We found that a Bacillus velezensis strain inhibits growth of and elicits NP production by Burkholderia thailandensis. Dereplication of known NPs detected in the metabolome of this B. velezensis strain suggests that a previously unannotated bioactive compound is involved. Thus, we present the use of whole-cell MALDI as a broadly applicable method for screening the NP composition of microbial co-cultures; this can be combined with other -omics methods to characterize probiotic-pathogen and other microbe-microbe interactions.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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