Kinetics of cone specific G-protein signaling in avian photoreceptor cells.
Autor: | Yee C; Division of Biochemistry, Department of Neuroscience, University of Oldenburg, Oldenburg, Germany., Görtemaker K; Division of Biochemistry, Department of Neuroscience, University of Oldenburg, Oldenburg, Germany., Wellpott R; Division of Biochemistry, Department of Neuroscience, University of Oldenburg, Oldenburg, Germany., Koch KW; Division of Biochemistry, Department of Neuroscience, University of Oldenburg, Oldenburg, Germany.; Research Center Neurosensory Sciences, University of Oldenburg, Oldenburg, Germany. |
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Jazyk: | angličtina |
Zdroj: | Frontiers in molecular neuroscience [Front Mol Neurosci] 2023 Jan 17; Vol. 16, pp. 1107025. Date of Electronic Publication: 2023 Jan 17 (Print Publication: 2023). |
DOI: | 10.3389/fnmol.2023.1107025 |
Abstrakt: | Cone photoreceptor cells of night-migratory songbirds seem to process the primary steps of two different senses, vision and magnetoreception. The molecular basis of phototransduction is a prototypical G protein-coupled receptor pathway starting with the photoexcitation of rhodopsin or cone opsin thereby activating a heterotrimeric G protein named transducin. This interaction is well understood in vertebrate rod cells, but parameter describing protein-protein interactions of cone specific proteins are rare and not available for migratory birds. European robin is a model organism for studying the orientation of birds in the earth magnetic field. Recent findings showed a link between the putative magnetoreceptor cryptochrome 4a and the cone specific G-protein of European robin. In the present work, we investigated the interaction of European robin cone specific G protein and cytoplasmic regions of long wavelength opsin. We identified the second loop in opsin connecting transmembrane regions three and four as a critical binding interface. Surface plasmon resonance studies using a synthetic peptide representing the second cytoplasmic loop and purified G protein α -subunit showed a high affinity interaction with a K Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Yee, Görtemaker, Wellpott and Koch.) |
Databáze: | MEDLINE |
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