Optical biosensor based on SERS with signal calibration function for quantitative detection of carcinoembryonic antigen.

Autor:	Wang T; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.; These authors contributed equally to this work., Zhu Y; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.; These authors contributed equally to this work., Weng S; Department of Thyroid and Breast Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China., Lin X; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.; Fujian Provincial Key Laboratory for Advanced Micro-nano Photonics Technology and Devices, Research Center for Photonics Technology, Quanzhou Normal University, Quanzhou, China., Kong KV; Department of Chemistry, National Taiwan University, Taipei, Taiwan., Weng Y; Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China., Jia X; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China., Chen R; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China., Lin D; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.; duo@fjnu.edu.cn., Feng S; Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, Fujian, China.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2022 Oct 20; Vol. 13 (11), pp. 5962-5970. Date of Electronic Publication: 2022 Oct 20 (Print Publication: 2022).
DOI:	10.1364/BOE.474273
Abstrakt:	Monitoring the levels of cancer biomarkers is essential for cancer diagnosis and evaluation. In this study, a novel sandwich type sensing platform based on surface-enhanced Raman scattering (SERS) technology was developed for the detection of carcinoembryonic antigen (CEA), with a limit of detection (LOD) of 0.258 ng/mL. In order to achieve sensitive detection of CEA in complex samples, gold nanoparticle monolayer modified with CEA antibodies and with aptamer-functionalized probes was fabricated to target CEA. Two gold layers were integrated into the SERS platform, which greatly enhanced the signal of the probe by generating tremendous "hot spots". Meanwhile, the intensity ratio of Raman probes and the second-order peak of the silicon wafer was used to achieve dynamic calibration of the Raman probe signal. Excitingly, this sensing platform was capable of distinguishing cancer patients from healthy individuals via CEA concentrations in blood samples with the accuracy of 100%. This sandwich structure SERS sensing platform presented promising potential to be an alternative tool for clinical biomarker detection in the field of cancer diagnosis. Competing Interests: The authors declare that there are no conflicts of interest related to this article. (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)
Databáze:	MEDLINE
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