Mapping thalamic innervation to individual L2/3 pyramidal neurons and modeling their 'readout' of visual input.

Autor: Balcioglu A; Picower Institute for Learning and Memory, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Gillani R; Picower Institute for Learning and Memory, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Neurology, Massachusetts General Hospital, Boston, MA, USA., Doron M; The Edmond and Lily Safra Center for Brain Sciences, Jerusalem, Israel.; Department of Neurobiology, The Hebrew University of Jerusalem, Jerusalem, Israel.; Broad Institute of Harvard University and MIT, Cambridge, MA, USA., Burnell K; Picower Institute for Learning and Memory, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Ku T; Picower Institute for Learning and Memory, Cambridge, MA, USA.; Institute for Medical Engineering and Science, Cambridge, MA, USA.; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea., Erisir A; Department of Psychology, University of Virginia, Charlottesville, VA, USA., Chung K; Picower Institute for Learning and Memory, Cambridge, MA, USA.; Department of Neurobiology, The Hebrew University of Jerusalem, Jerusalem, Israel.; Institute for Medical Engineering and Science, Cambridge, MA, USA.; Broad Institute of Harvard University and MIT, Cambridge, MA, USA., Segev I; The Edmond and Lily Safra Center for Brain Sciences, Jerusalem, Israel.; Department of Neurobiology, The Hebrew University of Jerusalem, Jerusalem, Israel., Nedivi E; Picower Institute for Learning and Memory, Cambridge, MA, USA. nedivi@mit.edu.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. nedivi@mit.edu.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. nedivi@mit.edu.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2023 Mar; Vol. 26 (3), pp. 470-480. Date of Electronic Publication: 2023 Feb 02.
DOI: 10.1038/s41593-022-01253-9
Abstrakt: The thalamus is the main gateway for sensory information from the periphery to the mammalian cerebral cortex. A major conundrum has been the discrepancy between the thalamus's central role as the primary feedforward projection system into the neocortex and the sparseness of thalamocortical synapses. Here we use new methods, combining genetic tools and scalable tissue expansion microscopy for whole-cell synaptic mapping, revealing the number, density and size of thalamic versus cortical excitatory synapses onto individual layer 2/3 (L2/3) pyramidal cells (PCs) of the mouse primary visual cortex. We find that thalamic inputs are not only sparse, but remarkably heterogeneous in number and density across individual dendrites and neurons. Most surprising, despite their sparseness, thalamic synapses onto L2/3 PCs are smaller than their cortical counterparts. Incorporating these findings into fine-scale, anatomically faithful biophysical models of L2/3 PCs reveals how individual neurons with sparse and weak thalamocortical synapses, embedded in small heterogeneous neuronal ensembles, may reliably 'read out' visually driven thalamic input.
(© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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