Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity.

Autor: Gu Y; Microbial Sciences Institute, Yale University, West Haven, CT, USA. ygu@mrc-lmb.cam.ac.uk.; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA. ygu@mrc-lmb.cam.ac.uk.; PNAC division, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK. ygu@mrc-lmb.cam.ac.uk., Guberman-Pfeffer MJ; Microbial Sciences Institute, Yale University, West Haven, CT, USA.; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA., Srikanth V; Microbial Sciences Institute, Yale University, West Haven, CT, USA.; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA., Shen C; Microbial Sciences Institute, Yale University, West Haven, CT, USA.; Department of Microbiology, Yale University, New Haven, CT, USA., Giska F; Department of Cell Biology, Yale University, New Haven, CT, USA.; Nanobiology Institute, Yale University, West Haven, CT, USA., Gupta K; Department of Cell Biology, Yale University, New Haven, CT, USA.; Nanobiology Institute, Yale University, West Haven, CT, USA., Londer Y; Microbial Sciences Institute, Yale University, West Haven, CT, USA.; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA., Samatey FA; Microbial Sciences Institute, Yale University, West Haven, CT, USA.; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA., Batista VS; Department of Chemistry, Yale University, New Haven, CT, USA., Malvankar NS; Microbial Sciences Institute, Yale University, West Haven, CT, USA. nikhil.malvankar@yale.edu.; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA. nikhil.malvankar@yale.edu.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2023 Feb; Vol. 8 (2), pp. 284-298. Date of Electronic Publication: 2023 Feb 02.
DOI: 10.1038/s41564-022-01315-5
Abstrakt: OmcZ nanowires produced by Geobacter species have high electron conductivity (>30 S cm -1 ). Of 111 cytochromes present in G. sulfurreducens, OmcZ is the only known nanowire-forming cytochrome essential for the formation of high-current-density biofilms that require long-distance (>10 µm) extracellular electron transport. However, the mechanisms underlying OmcZ nanowire assembly and high conductivity are unknown. Here we report a 3.5-Å-resolution cryogenic electron microscopy structure for OmcZ nanowires. Our structure reveals linear and closely stacked haems that may account for conductivity. Surface-exposed haems and charge interactions explain how OmcZ nanowires bind to diverse extracellular electron acceptors and how organization of nanowire network re-arranges in different biochemical environments. In vitro studies explain how G. sulfurreducens employ a serine protease to control the assembly of OmcZ monomers into nanowires. We find that both OmcZ and serine protease are widespread in environmentally important bacteria and archaea, thus establishing a prevalence of nanowire biogenesis across diverse species and environments.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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