Iron inactivation by Sporobolomyces ruberrimus and its potential role in plant metal stress protection. An in vitro study.

Autor: Jędrzejczyk RJ; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: roman.jedrzejczyk@uj.edu.pl., Gustab M; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: maciej.gustab@doctoral.uj.edu.pl., Ważny R; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: rafal.wazny@uj.edu.pl., Domka A; W. Szafer Institute of Botany Polish Academy of Sciences, Lubicz 46, 31-512 Kraków, Poland. Electronic address: agnieszka.domka@uj.edu.pl., Jodłowski PJ; Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 30-155 Kraków, Poland. Electronic address: przemyslaw.jodlowski@pk.edu.pl., Sitarz M; Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza 30, 30-059 Kraków, Poland. Electronic address: msitarz@agh.edu.pl., Bezkosty P; Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza 30, 30-059 Kraków, Poland. Electronic address: bezkosty@agh.edu.pl., Kowalski M; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: m.kowalski@doctoral.uj.edu.pl., Pawcenis D; Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland. Electronic address: d.pawcenis@uj.edu.pl., Jarosz K; Institute of Geological Sciences, Jagiellonian University, Gronostajowa 3a, 30-387 Kraków, Poland. Electronic address: kinga.borek@uj.edu.pl., Sebastian V; Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Zaragoza, Spain; Department of Chemical and Environmental Engineering, Universidad de Zaragoza, Campus Rio Ebro, 50018 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain; Laboratorio de Microscopías Avanzadas, Universidad de Zaragoza, 50018 Zaragoza, Spain., Łabaj PP; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: pawel.labaj@uj.edu.pl., Rozpądek P; Małopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland. Electronic address: piotr.rozpadek@uj.edu.pl.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Apr 20; Vol. 870, pp. 161887. Date of Electronic Publication: 2023 Jan 30.
DOI: 10.1016/j.scitotenv.2023.161887
Abstrakt: The endophytic Basidiomycete Sporobolomyces ruberrimus protects its host Arabidopsis arenosa against metal toxicity. Plants inoculated with the fungus yielded more biomass and exhibited significantly fewer stress symptoms in medium mimicking mine dump conditions (medium supplemented with excess of Fe, Zn and Cd). Aside from fine-tuning plant metal homeostasis, the fungus was capable of precipitating Fe in the medium, most likely limiting host exposure to metal toxicity. The precipitated residue was identified by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-Ray Diffraction (XRD) and electron microscopy (SEM/TEM) with energy dispersive X-Ray analysis (EDX/SAED) techniques. The performed analyses revealed that the fungus transforms iron into amorphous (oxy)hydroxides and phosphates and immobilizes them in the form of a precipitate changing Fe behaviour in the MSR medium. Moreover, the complexation of free Fe ions by fungi could be obtained by biomolecules such as lipids, proteins, or biosynthesized redox-active molecules.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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