The Formation and Displacement of Ordered DNA Triplexes in Self-Assembled Three-Dimensional DNA Crystals.

Autor:	Zhao Y; Department of Chemistry, New York University, New York, New York 10003, United States., Chandrasekaran AR; The RNA Institute, University of Albany, State University of New York, Albany, New York 12222, United States., Rusling DA; School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, U.K., Woloszyn K; Department of Chemistry, New York University, New York, New York 10003, United States., Hao Y; Department of Chemistry, New York University, New York, New York 10003, United States., Hernandez C; Department of Chemistry, New York University, New York, New York 10003, United States., Vecchioni S; Department of Chemistry, New York University, New York, New York 10003, United States., Ohayon YP; Department of Chemistry, New York University, New York, New York 10003, United States., Mao C; Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States., Seeman NC; Department of Chemistry, New York University, New York, New York 10003, United States., Sha R; Department of Chemistry, New York University, New York, New York 10003, United States.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2023 Feb 15; Vol. 145 (6), pp. 3599-3605. Date of Electronic Publication: 2023 Feb 02.
DOI:	10.1021/jacs.2c12667
Abstrakt:	Reconfigurable structures engineered through DNA hybridization and self-assembly offer both structural and dynamic applications in nanotechnology. Here, we have demonstrated that strand displacement of triplex-forming oligonucleotides (TFOs) can be translated to a robust macroscopic DNA crystal by coloring the crystals with covalently attached fluorescent dyes. We show that three different types of triplex strand displacement are feasible within the DNA crystals and the bound TFOs can be removed and/or replaced by (a) changing the pH from 5 to 7, (b) the addition of the Watson-Crick complement to a TFO containing a short toehold, and (c) the addition of a longer TFO that uses the duplex edge as a toehold. We have also proved by X-ray diffraction that the structure of the crystals remains as designed in the presence of the TFOs.
Databáze:	MEDLINE
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