Influence of Transforming Growth Factors beta 1 and beta 3 in the Scar Formation Process.

Autor:	Lee JS; Department of Plastic and Reconstructive Surgery., Cho HG; Department of Plastic and Reconstructive Surgery., Lee JW; Department of Plastic and Reconstructive Surgery., Oh EJ; Department of Plastic and Reconstructive Surgery.; Cell and Matrix Research Institute, School of Medicine, Kyungpook National University., Kim HM; Department of Plastic and Reconstructive Surgery.; Cell and Matrix Research Institute, School of Medicine, Kyungpook National University., Ko UH; Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology., Kang M; Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology., Shin JH; Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology., Chung HY; Department of Plastic and Reconstructive Surgery.; Cell and Matrix Research Institute, School of Medicine, Kyungpook National University.; Kyungpook National University Bio-Medical Research Institute, Kyungpook National University, Kyungpook National University Hospital, Korea.
Jazyk:	angličtina
Zdroj:	The Journal of craniofacial surgery [J Craniofac Surg] 2023 May 01; Vol. 34 (3), pp. 904-909. Date of Electronic Publication: 2022 Nov 01.
DOI:	10.1097/SCS.0000000000009087
Abstrakt:	Background: Transforming growth factor-beta (TGF-β) plays an instrumental role in forming scars and keloids. TGF-β isoforms exhibit differential expression, indicating distinct wound healing and scar formation functions. However, the role of TGF-β1 and TGF-β3 in wound healing and scar formation remains unclear. This study aimed to compare the specific roles of TGF-β1 and TGF-β3 in wound healing and scar formation by biomolecular analysis. Materials and Methods: The study was conducted by cell isolation and culture cells from a total of 20 human samples. Normal human fibroblasts (NHF) were isolated from normal human samples and myofibroblasts from the different scar types, namely hypertrophic (HT) and keloid (K) scars. NHF and cells from the HT, and K scar, each of which were divided into 3 sample groups: the untreated control, TGF-β1 (10 µg/mL)-treated group, and TGF-β3 (10 µg/mL)-treated group. The results of confocal microscopy and fluorescence-activated cell sorting experiments were compared. Results: Both the HT and K groups had higher α-smooth muscle actin (α-SMA) expression than the NHF group in the untreated control group. In comparison with the untreated group, NHFs showed a significant increase in α-SMA expression in the TGF-β1-treated group. HT showed a high α-SMA level, which was statistically significant compared with the normal fibroblasts. In the TGF-β3-treated group, α-SMA expression was slightly increased in NHF as compared with the untreated group. TGF-β3 treated HT exhibited a greater reduction in α-SMA expression than in the TGF-β1 treated HT. K, on the other hand, had only a minimal effect on the treatment of TGF-β1 and TGF-β3. Conclusions: The findings suggest that TGF-β3 may play a regulatory role in the wound repair process, which could be useful in the development of scar-reducing therapies for patients with scar-related cosmetic concerns. Competing Interests: The authors report no conflicts of interest. (Copyright © 2022 by Mutaz B. Habal, MD.)
Databáze:	MEDLINE
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