Evolutionary tuning of barbed end competition allows simultaneous construction of architecturally distinct actin structures.
| Autor: | Wirshing ACE; Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University , Waltham, MA, USA., Rodriguez SG; Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University , Waltham, MA, USA., Goode BL; Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University , Waltham, MA, USA. |
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| Jazyk: | angličtina |
| Zdroj: | The Journal of cell biology [J Cell Biol] 2023 Apr 03; Vol. 222 (4). Date of Electronic Publication: 2023 Feb 02. |
| DOI: | 10.1083/jcb.202209105 |
| Abstrakt: | How cells simultaneously assemble actin structures of distinct sizes, shapes, and filamentous architectures is still not well understood. Here, we used budding yeast as a model to investigate how competition for the barbed ends of actin filaments might influence this process. We found that while vertebrate capping protein (CapZ) and formins can simultaneously associate with barbed ends and catalyze each other's displacement, yeast capping protein (Cap1/2) poorly displaces both yeast and vertebrate formins. Consistent with these biochemical differences, in vivo formin-mediated actin cable assembly was strongly attenuated by the overexpression of CapZ but not Cap1/2. Multiwavelength live cell imaging further revealed that actin patches in cap2∆ cells acquire cable-like features over time, including recruitment of formins and tropomyosin. Together, our results suggest that the activities of S. cerevisiae Cap1/2 have been tuned across evolution to allow robust cable assembly by formins in the presence of high cytosolic levels of Cap1/2, which conversely limit patch growth and shield patches from formins. (© 2023 Wirshing et al.) |
| Databáze: | MEDLINE |
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