Dissecting operant alcohol self-administration using saccharin-fading procedure.

Autor: Calleja-Conde J; Cardenal Cisneros Higher Education Center, Madrid, Spain., Echeverry-Alzate V; School of Life and Nature Sciences, Nebrija University, Madrid, Spain.; Department of Psychobiology and Methodology in Behavioral Sciences, Faculty of Psychology, Somosaguas Campus, Complutense University of Madrid, Madrid, Spain.; IMABIS Foundation, Regenerative Medicine Laboratory, Carlos Haya Regional University Hospital, Málaga, Spain., Bühler KM; Department of Psychobiology and Methodology in Behavioral Sciences, Faculty of Psychology, Somosaguas Campus, Complutense University of Madrid, Madrid, Spain., Morales-García JÁ; Department of Cell Biology, Faculty of Medicine, Complutense University of Madrid, Madrid, Spain., Segovia-Rodríguez L; Department of Psychobiology and Methodology in Behavioral Sciences, Faculty of Psychology, Somosaguas Campus, Complutense University of Madrid, Madrid, Spain., Durán-González P; Department of Psychobiology and Methodology in Behavioral Sciences, Faculty of Psychology, Somosaguas Campus, Complutense University of Madrid, Madrid, Spain., Olmos P; Center for Energy, Environmental and Technological Research (CIEMAT), Madrid, Spain., de Fonseca FR; IMABIS Foundation, Regenerative Medicine Laboratory, Carlos Haya Regional University Hospital, Málaga, Spain., Giné E; Department of Cell Biology, Faculty of Medicine, Complutense University of Madrid, Madrid, Spain., López-Moreno JA; Department of Psychobiology and Methodology in Behavioral Sciences, Faculty of Psychology, Somosaguas Campus, Complutense University of Madrid, Madrid, Spain.
Jazyk: angličtina
Zdroj: Neuropsychopharmacology reports [Neuropsychopharmacol Rep] 2023 Mar; Vol. 43 (1), pp. 12-22. Date of Electronic Publication: 2023 Feb 02.
DOI: 10.1002/npr2.12289
Abstrakt: Background: Although alcohol use disorder is a complex human pathology, the use of animal models represents an opportunity to study some aspects of this pathology. One of the most used paradigms to study the voluntary alcohol consumption in rodents is operant self-administration (OSA).
Aims: In order to facilitate the performance of this paradigm, we aim to describe some critical steps of OSA under a saccharin-fading procedure.
Material & Methods: We used 40 male Wistar rats to study the process of acquiring the operant response through a saccharin-fading procedure under a fixed ratio (FR1) schedule of reinforcement. Next, we analyze the alcohol introduction and concentration increase, the effect of an alcohol deprivation, and the analogy between this paradigm with the Drinking in the Dark-Multiple Scheduled Access paradigm.
Results: During alcohol concentration increase, animals reduced their lever presses in accordance with the increase in alcohol concentration. On the contrary, the consumption measured in g·kg -1 BW showed a great stability. The lever presses pattern within operant session changes with the introduction of different alcohol concentrations: at higher alcohol concentrations, animals tended to accumulate most of their presses in the initial period of the session.
Discussion: We show the utility of fading with low concentrations of saccharin and the evolution of the operant response through the different concentrations of alcohol.
Conclusion: Taken together, our results aimed to dissect the acquisition and maintenance of OSA behavior as well as other related variables, to facilitate the understanding and performance of this paradigm.
(© 2022 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
Databáze: MEDLINE
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