Evaluation of silk fibroin-based urinary conduits in a porcine model of urinary diversion.

Autor:	Gundogdu G; Department of Urology, University of California, Irvine, Orange, CA, United States., Nguyen T; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, United States., Hosseini Sharifi SH; Department of Urology, University of California, Irvine, Orange, CA, United States., Starek S; Department of Urology, University of California, Irvine, Orange, CA, United States., Costa K; Department of Urology, University of California, Irvine, Orange, CA, United States., Jones CE; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, United States., Barham D; Department of Urology, University of California, Irvine, Orange, CA, United States., Gelman J; Department of Urology, University of California, Irvine, Orange, CA, United States., Clayman RV; Department of Urology, University of California, Irvine, Orange, CA, United States., Mauney JR; Department of Urology, University of California, Irvine, Orange, CA, United States.; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2023 Jan 13; Vol. 11, pp. 1100507. Date of Electronic Publication: 2023 Jan 13 (Print Publication: 2023).
DOI:	10.3389/fbioe.2023.1100507
Abstrakt:	Background: The primary strategy for urinary diversion in radical cystectomy patients involves incorporation of autologous gastrointestinal conduits into the urinary tract which leads to deleterious consequences including chronic infections and metabolic abnormalities. This report investigates the efficacy of an acellular, tubular bi-layer silk fibroin (BLSF) graft to function as an alternative urinary conduit in a porcine model of urinary diversion. Materials and methods: Unilateral urinary diversion with stented BLSF conduits was executed in five adult female, Yucatan mini-swine over a 3 month period. Longitudinal imaging analyses including ultrasonography, retrograde ureteropyelography and video-endoscopy were carried out monthly. Histological, immunohistochemical (IHC), and histomorphometric assessments were performed on neoconduits at harvest. Results: All animals survived until scheduled euthanasia and displayed moderate hydronephrosis (Grades 1-3) in reconstructed collecting systems over the course of the study period. Stented BLSF constructs supported formation of vascularized, retroperitoneal tubes capable of facilitating external urinary drainage. By 3 months post-operative, neoconduits contained α -smooth muscle actin+ and SM22α+ smooth muscle as well as uroplakin 3A+ and pan-cytokeratin + urothelium. However, the degree of tissue regeneration in neotissues was significantly lower in comparison to ureteral controls as determined by histomorphometry. In addition, neoconduit stenting was necessary to prevent stomal occlusion. Conclusion: BLSF biomaterials represent emerging platforms for urinary conduit construction and may offer a functional replacement for conventional urinary diversion techniques following further optimization of mechanical properties and regenerative responses. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Gundogdu, Nguyen, Hosseini Sharifi, Starek, Costa, Jones, Barham, Gelman, Clayman and Mauney.)
Databáze:	MEDLINE
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