Continuously perfusable, customisable, and matrix-free vasculature on a chip platform.

Autor:	Chesnais F; Academic Centre of Reconstructive Science, Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. francois.chesnais@kcl.ac.uk., Joel J; Centre for Craniofacial and Regenerative Medicine, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK., Hue J; Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK., Shakib S; Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK., Di Silvio L; Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK., Grigoriadis AE; Centre for Craniofacial and Regenerative Medicine, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK., Coward T; Academic Centre of Reconstructive Science, Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. francois.chesnais@kcl.ac.uk., Veschini L; Academic Centre of Reconstructive Science, Centre for Oral, Clinical and Translational Sciences, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, Great Maze Pond, London SE1 9RT, UK. francois.chesnais@kcl.ac.uk.
Jazyk:	angličtina
Zdroj:	Lab on a chip [Lab Chip] 2023 Feb 14; Vol. 23 (4), pp. 761-772. Date of Electronic Publication: 2023 Feb 14.
DOI:	10.1039/d2lc00930g
Abstrakt:	Creating vascularised cellular environments in vitro is a current challenge in tissue engineering and a bottleneck towards developing functional stem cell-derived microtissues for regenerative medicine and basic investigations. Here we have developed a new workflow to manufacture vasculature on chip (VoC) systems efficiently, quickly, and inexpensively. We have employed 3D printing for fast-prototyping of bespoke VoC and coupled them with a refined organotypic culture system (OVAA) to grow patent capillaries in vitro using tissue-specific endothelial and stromal cells. Furthermore, we have designed and implemented a pocket-size flow driver to establish physiologic perfusive flow throughout our VoC-OVAA with minimal medium use and waste. Using our platform, we have created vascularised microtissues and perfused them at physiologic flow rates for extended time (>2 weeks) observing flow-dependent vascular remodelling. Overall, we present for the first time a scalable and customisable system to grow vascularised and perfusable microtissues, a key initial step to grow mature and functional tissues in vitro . We envision that this technology will empower fast prototyping and validation of increasingly biomimetic in vitro systems, including interconnected multi-tissue systems.
Databáze:	MEDLINE
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