| Autor: |
Sommariva M; Department of Biomedical Health for Science, Università degli Sudi di Milano, Milano, Italy., Busnelli M; Department of Pharmacological and Biomolecular Sciences, Università degli Sudi di Milano, Milano, Italy., Menegola E; Department of Environmental Science and Policy, Università degli Sudi di Milano, Milano, Italy., Renzo FD; Department of Environmental Science and Policy, Università degli Sudi di Milano, Milano, Italy., Indino S; Department of Biomedical Health for Science, Università degli Sudi di Milano, Milano, Italy., Menon A; Department of Biomedical Health for Science, Università degli Sudi di Milano, Milano, Italy.; Laboratory of Applied Biomechanics, Department of Biomedical Sciences for Health, University of Milan, Milano, Italy., Barajon I; Humanitas University, Milano, Italy., Arnaboldi F; Department of Biomedical Health for Science, Università degli Sudi di Milano, Milano, Italy. |
| Abstrakt: |
Toll-like receptors (TLRs) are the mammalian ortholog of Drosophila melanogaster protein Toll, originally identified for its involvement in embryonic development. In mammals, TLRs are mainly known for their ability to recognize pathogen- or damage-associated molecular patterns and, consequently, to initiate the immune response. However, it is becoming clear that TLRs can play a role also in mammal embryo development. We have previously described TLR4 and TLR7 expression in developing mouse peripheral nervous system and gastrointestinal tract. In the present study, we extended the investigation of TLR4 and TLR7 to the respiratory system and to the two main accessory organs of the digestive system, the liver and pancreas. TLR4 and TLR7 immunostaining was performed on mouse conceptuses collected at different stages, from E12 to E18. TLR4 and TLR7 immunoreactivity was evident in the embryo pancreas and liver at E12, while, in the respiratory apparatus, appeared at E14 and E17, respectively. Although further studies are required to elucidate the specific role of these TLRs in embryo development, the differential spatiotemporal TLR4 and TLR7 appearance may suggest that TLR expression in developing embryos is highly regulated for a possible their direct involvement in the formation of the organs and in the acquisition of immune-related features in preparation for the birth. |
