Single-molecule visualization of mRNA circularization during translation.

Autor: Kim B; Department of Physics, Pohang University of Science & Technology (POSTECH), Pohang, 37673, Republic of Korea., Seol J; School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang, 37673, Republic of Korea., Kim YK; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea., Lee JB; Department of Physics, Pohang University of Science & Technology (POSTECH), Pohang, 37673, Republic of Korea. jblee@postech.ac.kr.; School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang, 37673, Republic of Korea. jblee@postech.ac.kr.
Jazyk: angličtina
Zdroj: Experimental & molecular medicine [Exp Mol Med] 2023 Feb; Vol. 55 (2), pp. 283-289. Date of Electronic Publication: 2023 Jan 31.
DOI: 10.1038/s12276-023-00933-1
Abstrakt: Translation is mediated by precisely orchestrated sequential interactions among translation initiation components, mRNA, and ribosomes. Biochemical, structural, and genetic techniques have revealed the fundamental mechanism that determines what occurs and when, where and in what order. Most mRNAs are circularized via the eIF4E-eIF4G-PABP interaction, which stabilizes mRNAs and enhances translation by recycling ribosomes. However, studies using single-molecule fluorescence imaging have allowed for the visualization of complex data that opposes the traditional "functional circularization" theory. Here, we briefly introduce single-molecule techniques applied to studies on mRNA circularization and describe the results of in vitro and live-cell imaging. Finally, we discuss relevant insights and questions gained from single-molecule research related to translation.
(© 2023. The Author(s).)
Databáze: MEDLINE
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