Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza.
| Autor: | Wu T; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.; VIB Center for Plant Systems Biology, VIB, Ghent, Belgium., Natran A; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.; VIB Center for Plant Systems Biology, VIB, Ghent, Belgium., Prost L; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.; VIB Center for Plant Systems Biology, VIB, Ghent, Belgium.; Department of Biology, Ghent University, Ghent, Belgium., Aydogdu E; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.; VIB Center for Plant Systems Biology, VIB, Ghent, Belgium., Van de Peer Y; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium., Bafort Q; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. quinten.bafort@psb.ugent.be.; VIB Center for Plant Systems Biology, VIB, Ghent, Belgium. quinten.bafort@psb.ugent.be.; Department of Biology, Ghent University, Ghent, Belgium. quinten.bafort@psb.ugent.be. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2545, pp. 373-390. |
| DOI: | 10.1007/978-1-0716-2561-3_19 |
| Abstrakt: | In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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