Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee.

Autor: Carcaud J; Evolution, Genomes, Behavior and Ecology, Université Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France., Otte M; Evolutionnary Genetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Grünewald B; Institut für Bienenkunde, Polytechnische Gesellschaft, FB Biowissenschaften, Goethe-University, Frankfurt am Main, Germany., Haase A; Center for Mind/Brain Sciences (CIMeC), University of Trento, Rovereto, Italy.; Department of Physics, University of Trento, Trento, Italy., Sandoz JC; Evolution, Genomes, Behavior and Ecology, Université Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France., Beye M; Evolutionnary Genetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2023 Jan 31; Vol. 21 (1), pp. e3001984. Date of Electronic Publication: 2023 Jan 31 (Print Publication: 2023).
DOI: 10.1371/journal.pbio.3001984
Abstrakt: Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Carcaud et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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