The transcription factor VviNAC60 regulates senescence- and ripening-related processes in grapevine.

Autor:	D'Incà E; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Foresti C; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Orduña L; Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna 46908, Valencia, Spain., Amato A; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Vandelle E; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Santiago A; Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna 46908, Valencia, Spain., Botton A; Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova 35122, Italy., Cazzaniga S; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Bertini E; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Pezzotti M; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Giovannoni JJ; USDA-ARS Robert W. Holley Center and Boyce Thompson Institute for Plant Research, Tower Road, Cornell Campus, Ithaca, NY 14853, USA., Vrebalov JT; USDA-ARS Robert W. Holley Center and Boyce Thompson Institute for Plant Research, Tower Road, Cornell Campus, Ithaca, NY 14853, USA., Matus JT; Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna 46908, Valencia, Spain., Tornielli GB; Department of Biotechnology, University of Verona, 37134 Verona, Italy., Zenoni S; Department of Biotechnology, University of Verona, 37134 Verona, Italy.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2023 Jul 03; Vol. 192 (3), pp. 1928-1946.
DOI:	10.1093/plphys/kiad050
Abstrakt:	Grapevine (Vitis vinifera L.) is one of the most widely cultivated fruit crops because the winemaking industry has huge economic relevance worldwide. Uncovering the molecular mechanisms controlling the developmental progression of plant organs will prove essential for maintaining high-quality grapes, expressly in the context of climate change, which impairs the ripening process. Through a deep inspection of transcriptomic data, we identified VviNAC60, a member of the NAC transcription factor family, as a putative regulator of grapevine organ maturation. We explored VviNAC60 binding landscapes through DNA affinity purification followed by sequencing and compared bound genes with transcriptomics datasets from grapevine plants stably and transiently overexpressing VviNAC60 to define a set of high-confidence targets. Among these, we identified key molecular markers associated with organ senescence and fruit ripening. Physiological, metabolic, and promoter activation analyses showed that VviNAC60 induces chlorophyll degradation and anthocyanin accumulation through the upregulation of STAY-GREEN PROTEIN 1 (VviSGR1) and VviMYBA1, respectively, with the latter being upregulated through a VviNAC60-VviNAC03 regulatory complex. Despite sharing a closer phylogenetic relationship with senescence-related homologs to the NAC transcription factor AtNAP, VviNAC60 complemented the nonripening(nor) mutant phenotype in tomato (Solanum lycopersicum), suggesting a dual role as an orchestrator of both ripening- and senescence-related processes. Our data support VviNAC60 as a regulator of processes initiated in the grapevine vegetative- to mature-phase organ transition and therefore as a potential target for enhancing the environmental resilience of grapevine by fine-tuning the duration of the vegetative phase. Competing Interests: Conflict of interest statement. The authors declare that they have no conflict of interest. (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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