Methods to Measure Water Permeability.
| Autor: | Solenov EI; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia. eugsol@bionet.nsc.ru.; Novosibirsk State Technical University, Novosibirsk, Russia. eugsol@bionet.nsc.ru., Baturina GS; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia., Katkova LE; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia., Yang B; School of Basic Medical Sciences, Peking University, Beijing, China., Zarogiannis SG; Department of Physiology, Faculty of Medicine, University of Thessaly, BIOPOLIS, Larissa, Greece. |
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| Jazyk: | angličtina |
| Zdroj: | Advances in experimental medicine and biology [Adv Exp Med Biol] 2023; Vol. 1398, pp. 343-361. |
| DOI: | 10.1007/978-981-19-7415-1_24 |
| Abstrakt: | Water permeability is a key feature of the cell plasma membranes, and it has seminal importance for several cell functions such as cell volume regulation, cell proliferation, cell migration, and angiogenesis to name a few. The transport of water occurs mainly through plasma membrane water channels, aquaporins. Aquaporins have very important function in physiological and pathophysiological states. Due to the above, the experimental assessment of the water permeability of cells and tissues is necessary. The development of new methodologies of measuring water permeability is a vibrant scientific field that constantly develops during the last three decades along with the advances in imaging mainly. In this chapter we describe and critically assess several methods that have been developed for the measurement of water permeability both in living cells and in tissues with a focus in the first category. (© 2023. The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.) |
| Databáze: | MEDLINE |
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