Understanding ayahuasca effects in major depressive disorder treatment through in vitro metabolomics and bioinformatics.

Autor: Zandonadi FS; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Silva AAR; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University (USF), Bragança Paulista, SP, 12916-900, Brazil., Melo AAS; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Ignarro RS; Laboratory of Nerve Regeneration, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Matos TS; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Santos EAF; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Barbosa LD; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Oliveira ALR; Laboratory of Nerve Regeneration, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil., Porcari AM; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University (USF), Bragança Paulista, SP, 12916-900, Brazil., Sussulini A; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil. sussulini@unicamp.br.; Institute of Chemistry, Instituto Nacional de Ciência E Tecnologia Em Bioanalítica (INCTBio), University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil. sussulini@unicamp.br.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2023 Jul; Vol. 415 (18), pp. 4367-4384. Date of Electronic Publication: 2023 Jan 30.
DOI: 10.1007/s00216-023-04556-3
Abstrakt: Emerging insights from metabolomic-based studies of major depression disorder (MDD) are mainly related to biochemical processes such as energy or oxidative stress, in addition to neurotransmission linked to specific metabolite intermediates. Hub metabolites represent nodes in the biochemical network playing a critical role in integrating the information flow in cells between metabolism and signaling pathways. Limited technical-scientific studies have been conducted to understand the effects of ayahuasca (Aya) administration in the metabolism considering MDD molecular context. Therefore, this work aims to investigate an in vitro primary astrocyte model by untargeted metabolomics of two cellular subfractions: secretome and intracellular content after pre-defined Aya treatments, based on DMT concentration. Mass spectrometry (MS)-based metabolomics data revealed significant hub metabolites, which were used to predict biochemical pathway alterations. Branched-chain amino acid (BCAA) metabolism, and vitamin B6 and B3 metabolism were associated to Aya treatment, as "housekeeping" pathways. Dopamine synthesis was overrepresented in the network results when considering the lowest tested DMT concentration (1 µmol L -1 ). Building reaction networks containing significant and differential metabolites, such as nicotinamide, L-DOPA, and L-leucine, is a useful approach to guide on dose decision and pathway selection in further analytical and molecular studies.
(© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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