A cluster of broadly neutralizing IgG against BK polyomavirus in a repertoire dominated by IgM.

Autor:	Nguyen NK; Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France., Devilder MC; Nantes Université, Inserm UMR 1307, CNRS UMR 6075, Université d'Angers, CRCI2NA, Nantes, France., Gautreau-Rolland L; Nantes Université, Inserm UMR 1307, CNRS UMR 6075, Université d'Angers, CRCI2NA, Nantes, France.; UFR Sciences et Techniques, Nantes Université, Nantes, France., Fourgeux C; Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France., Sinha D; Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France., Poschmann J; Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France., Hourmant M; CHU Nantes, Nantes Université, Service de Néphrologie-Immunologie clinique, Nantes, France., Bressollette-Bodin C; Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France.; CHU Nantes, Nantes Université, Service de Virologie, Nantes, France.; UFR Médecine, Nantes Université, Nantes, France., Saulquin X; Nantes Université, Inserm UMR 1307, CNRS UMR 6075, Université d'Angers, CRCI2NA, Nantes, France xavier.saulquin@univ-nantes.fr dorian.mcilroy@univ-nantes.fr.; UFR Sciences et Techniques, Nantes Université, Nantes, France., McIlroy D; Nantes Université,, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ITUN, Nantes, France xavier.saulquin@univ-nantes.fr dorian.mcilroy@univ-nantes.fr.; UFR Sciences et Techniques, Nantes Université, Nantes, France.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2023 Jan 30; Vol. 6 (4). Date of Electronic Publication: 2023 Jan 30 (Print Publication: 2023).
DOI:	10.26508/lsa.202201567
Abstrakt:	The BK polyomavirus (BKPyV) is an opportunistic pathogen, which is only pathogenic in immunosuppressed individuals, such as kidney transplant recipients, in whom BKPyV can cause significant morbidity. To identify broadly neutralizing antibodies against this virus, we used fluorescence-labeled BKPyV virus-like particles to sort BKPyV-specific B cells from the PBMC of KTx recipients, then single-cell RNAseq to obtain paired heavy- and light-chain antibody sequences from 2,106 sorted B cells. The BKPyV-specific repertoire was highly diverse in terms of both V-gene usage and clonotype diversity and included most of the IgM B cells, including many with extensive somatic hypermutation. In two patients where sufficient data were available, IgM B cells in the BKPyV-specific dataset had significant differences in V-gene usage compared with IgG B cells from the same patient. CDR3 sequence-based clustering allowed us to identify and characterize three broadly neutralizing "41F17-like" clonotypes that were predominantly IgG, suggesting that some specific BKPyV capsid epitopes are preferentially targeted by IgG. (© 2023 Nguyen et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu