An IS-mediated, RecA-dependent, bet-hedging strategy in Burkholderia thailandensis .

Autor:	Lowrey LC; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, United States., Kent LA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, United States., Rios BM; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, United States., Ocasio AB; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, United States., Cotter PA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Jan 30; Vol. 12. Date of Electronic Publication: 2023 Jan 30.
DOI:	10.7554/eLife.84327
Abstrakt:	Adaptation to fluctuating environmental conditions is difficult to achieve. Phase variation mechanisms can overcome this difficulty by altering genomic architecture in a subset of individuals, creating a phenotypically heterogeneous population with subpopulations optimized to persist when conditions change, or are encountered, suddenly. We have identified a phase variation system in Burkholderia thailandensis that generates a genotypically and phenotypically heterogeneous population. Genetic analyses revealed that RecA-mediated homologous recombination between a pair of insertion sequence (IS) 2 -like elements duplicates a 208.6 kb region of DNA that contains 157 coding sequences. RecA-mediated homologous recombination also resolves merodiploids, and hence copy number of the region is varied and dynamic within populations. We showed that the presence of two or more copies of the region is advantageous for growth in a biofilm, and a single copy is advantageous during planktonic growth. While IS elements are well known to contribute to evolution through gene inactivation, polar effects on downstream genes, and altering genomic architecture, we believe that this system represents a rare example of IS element-mediated evolution in which the IS elements provide homologous sequences for amplification of a chromosomal region that provides a selective advantage under specific growth conditions, thereby expanding the lifestyle repertoire of the species. Competing Interests: LL, LK, BR, AO, PC No competing interests declared (© 2023, Lowrey et al.)
Databáze:	MEDLINE
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