Comparing lipid remodeling of brown adipose tissue, white adipose tissue, and liver after one-week high fat diet intervention with quantitative Raman microscopy.

Autor: Paul A; Division of Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.; Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA., Chanclón B; Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Brännmark C; Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Wittung-Stafshede P; Division of Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden., Olofsson CS; Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Asterholm IW; Department of Physiology (Metabolic Physiology), Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Parekh SH; Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA.; Department of Molecular Spectroscopy, Max Planck Institute for Polymer Research, Mainz, Germany.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2023 Mar; Vol. 124 (3), pp. 382-395. Date of Electronic Publication: 2023 Jan 30.
DOI: 10.1002/jcb.30372
Abstrakt: Brown adipose tissue (BAT) consists of highly metabolically active adipocytes that catabolize nutrients to produce heat. Playing an active role in triacylglycerol (TAG) clearance, research has shown that dietary fatty acids can modulate the TAG chemistry deposition in BAT after weeks-long dietary intervention, similar to what has been shown in white adipose tissue (WAT). Our objective was to compare the influence of sustained, nonchronic dietary intervention (a 1-week interval) on WAT and interscapular BAT lipid metabolism and deposition in situ. We use quantitative, label-free chemical microscopy to show that 1 week of high fat diet (HFD) intervention results in dramatically larger lipid droplet (LD) growth in BAT (and liver) compared to LD growth in inguinal WAT (IWAT). Moreover, BAT showed lipid remodeling as increased unsaturated TAGs in LDs, resembling the dietary lipid composition, while WAT (and liver) did not show lipid remodeling on this time scale. Concurrently, expression of genes involved in lipid metabolism, particularly desaturases, was reduced in BAT and liver from HFD-fed mice after 1 week. Our data show that BAT lipid chemistry remodels exceptionally fast to dietary lipid intervention compared WAT, which further points towards a role in TAG clearance.
(© 2023 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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