Mosquito tagging using DNA-barcoded nanoporous protein microcrystals.

Autor:	Stuart JD; Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA., Hartman DA; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.; Department of Entomology, Cornell University, Ithaca, NY 14853, USA., Gray LI; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA., Jones AA; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA., Wickenkamp NR; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA., Hirt C; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA., Safira A; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA., Regas AR; College of Veterinary Medicine and Biological Sciences, Colorado State University, Fort Collins, CO 80523, USA., Kondash TM; Department of Environmental Health and Radiological Sciences, Colorado State University, Fort Collins, CO 80523, USA.; H3 Environmental, Albuquerque, NM 87109 (current)., Yates ML; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA., Driga S; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, USA., Snow CD; Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado 80523, USA., Kading RC
Jazyk:	angličtina
Zdroj:	PNAS nexus [PNAS Nexus] 2022 Sep 12; Vol. 1 (4), pp. pgac190. Date of Electronic Publication: 2022 Sep 12 (Print Publication: 2022).
DOI:	10.1093/pnasnexus/pgac190
Abstrakt:	Conventional mosquito marking technology for mark-release-recapture (MRR) is quite limited in terms of information capacity and efficacy. To overcome both challenges, we have engineered, lab-tested, and field-evaluated a new class of marker particles, in which synthetic, short DNA oligonucleotides (DNA barcodes) are adsorbed and protected within tough, crosslinked porous protein microcrystals. Mosquitoes self-mark through ingestion of microcrystals in their larval habitat. Barcoded microcrystals persist trans-stadially through mosquito development if ingested by larvae, do not significantly affect adult mosquito survivorship, and individual barcoded mosquitoes are detectable in pools of up to at least 20 mosquitoes. We have also demonstrated crystal persistence following adult mosquito ingestion. Barcode sequences can be recovered by qPCR and next-generation sequencing (NGS) without detectable amplification of native mosquito DNA. These DNA-laden protein microcrystals have the potential to radically increase the amount of information obtained from future MRR studies compared to previous studies employing conventional mosquito marking materials. (© The Author(s) 2022. Published by Oxford University Press on behalf of National Academy of Sciences.)
Databáze:	MEDLINE
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