Expression of collagenases (matrix metalloproteinase-1, -8, -13) and tissue inhibitor of metalloproteinase-3 (TIMP-3) in naturally occurring bovine cutaneous fibropapillomas.

Autor:	Daraban Bocaneti F; Department of Public Health, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Altamura G; Department of Veterinary Medicine and Animal Productions, University of Naples 'Federico II', Naples, Italy., Corteggio A; Institute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), Naples, Italy., Tanase OI; Department of Public Health, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Dascalu MA; Department of Public Health, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Pasca SA; Department of Pathology, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Hritcu O; Department of Pathology, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Mares M; Department of Public Health, Faculty of Veterinary Medicine, Iasi University of Life Sciences 'Ion Ionescu de la Brad', Iaşi, Romania., Borzacchiello G; Department of Veterinary Medicine and Animal Productions, University of Naples 'Federico II', Naples, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Jan 12; Vol. 9, pp. 1072672. Date of Electronic Publication: 2023 Jan 12 (Print Publication: 2022).
DOI:	10.3389/fvets.2022.1072672
Abstrakt:	Bovine cutaneous fibropapillomas are among the most common skin tumors in cattle; their etiology is associated with infection by bovine papillomavirus (BPV) types-1/-2 which are considered oncogenic. Degradation of the extracellular matrix (ECM), especially collagenolysis, is a key event during a series of relevant physiological processes, including tissue remodeling and repair. Various types of proteins are implicated in the regulation of ECM degradation: among these, matrix metalloproteinases (MMPs), a group of zinc-dependent endoenzymes, and tissue inhibitors of matrix metalloproteinases (TIMPs) are known to play a major role. Previous studies reported that aberrant expression of collagenolytic MMPs (MMP-1/-8/-13) and unbalancing between MMPs and TIMPs represent a critical step in tumor growth and invasion; however, studies regarding this topic in bovine cutaneous fibropapillomas are lacking. The aim of this study was to investigate the expression of the collagenases MMP-1/-8/-13 and TIMP-3 in naturally occurring fibropapillomas harboring BPV-2 DNA and normal skin samples. Here, by immunohistochemistry and western blotting analysis, we demonstrated overexpression of MMP-8/-13 along with a down-regulation of MMP-1, associated with a decrease in TIMP-3 levels in tumor compared with normal skin samples. This is the first study describing MMP-1/-8/-13 and TIMP-3 expression in bovine cutaneous fibropapillomas and our results suggest that an impaired expression of collagenases along with an imbalance between MMPs/TIMPs may contribute to an increased collagenolytic activity, which in turn could be important in ECM changes and tumors development. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Daraban Bocaneti, Altamura, Corteggio, Tanase, Dascalu, Pasca, Hritcu, Mares and Borzacchiello.)
Databáze:	MEDLINE
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