Increased expression of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for growth of mouse embryonic stem cells that are undergoing differentiation.

Autor: Guzel S; Department of Biochemistry, School of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey., Gurpinar Y; Research Center for Translational Medicine, Koc University, 34010 Istanbul, Turkey., Altunok TH; Department of Biochemistry, School of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey., Yalcin A; Department of Biochemistry, School of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey.
Jazyk: angličtina
Zdroj: Cytotechnology [Cytotechnology] 2023 Feb; Vol. 75 (1), pp. 27-38. Date of Electronic Publication: 2022 Nov 10.
DOI: 10.1007/s10616-022-00557-9
Abstrakt: The unlimited proliferation capacity of embryonic stem cells (ESCs) coupled with their capability to differentiate into several cell types makes them an attractive candidate for studying the molecular mechanisms regulating self-renewal and transition from pluripotent state. Although the roles of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase family (PFKFB1-4) in cell survival, proliferation, and differentiation in tumor cells have been studied, their role in mouse ESC (mESC) biology is currently unkown. In the current study, Pfkfb isoenzyme expressions were analyzed in R1 and J1 mESCs that were cultured in the presence and absence of leukemia inhibitory factor (LIF). We report that expression of the Pfkfb3 isoenzyme was markedly increased when mESCs were promoted to differentiate upon LIF removal. We then demonstrated that Pfkfb3 silencing induced the differentiation marker Brachyury suggesting that Pfkfb3 may be required for the regulation of mesodermal differentiation of mESCs. Furthermore, we show that the increase in Pfkfb3 expression is required for the growth of early differentiated mESCs. Although these results provide important insights into the early differentiation of mESCs with regard to Pfkfb expressions, further mechanistic studies will be needed for understanding the pathways and mechanisms involved in regulation of proliferation and early differentiation of mESCs through Pfkfb3.
Competing Interests: Competing interestsThe authors declare that they have no relevant financial or non-financial interests to disclose.
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Databáze: MEDLINE
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