Exploring cell surface markers and cell-cell interactions of human breast milk stem cells.

Autor:	Coni P; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Piras M; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Piludu M; Department of Biomedical Sciences, University of Cagliari, Cagliari, Italy., Lachowicz JI; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Matteddu A; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Coni S; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Reali A; Azienda Ospedaliero Universitaria di Cagliari, Terapia Intesiva Neonatale (TIN), P.O. Duilio Casula di Monserrato, Cagliari, Italy., Fanos V; Department of Surgical Sciences, University of Cagliari, Cagliari, Italy., Jaremko M; Smart-Health Initiative (SHI) and Red Sea Research Center (RSRC), Division of Biological and Environ-mental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Faa G; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy., Pichiri G; Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy.
Jazyk:	angličtina
Zdroj:	Journal of public health research [J Public Health Res] 2023 Jan 24; Vol. 12 (1), pp. 22799036221150332. Date of Electronic Publication: 2023 Jan 24 (Print Publication: 2023).
DOI:	10.1177/22799036221150332
Abstrakt:	Background: Breakthrough studies have shown that pluripotent stem cells are present in human breast milk. The expression of pluripotency markers by breast milk cells is heterogeneous, relating to cellular hierarchy, from early-stage multi-lineage stem cells to fully differentiated mammary epithelial cells, as well as weeks of gestation and days of lactation. Design and Methods: Here, we qualitatively analyze cell marker expression in freshly isolated human breast milk cells, without any manipulation that could influence protein expression. Moreover, we use electron microscopy to investigate cell-cell networks in breast milk for the first time, providing evidence of active intercellular communication between cells expressing different cellular markers. Results: The immunocytochemistry results of human breast milk cells showed positive staining in all samples for CD44, CD45, CD133, and Ki67 markers. Variable positivity was present with P63, Tβ4 and CK14 markers. No immunostaining was detected for Wt1, nestin, Nanog, OCT4, SOX2, CK5, and CD34 markers. Cells isolated from human breast milk form intercellular connections, which together create a cell-to-cell communication network. Conclusions: Cells freshly isolated form human breast milk, without particular manipulations, show heterogeneous expression of stemness markers. The studied milk staminal cells show "pluripotency" at different stages of differentiation, and are present as single cells or grouped cells. The adjacent cell interactions are evidenced by electron microscopy, which showed the formation of intercellular connections, numerous contact regions, and thin pseudopods. Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. (© The Author(s) 2023.)
Databáze:	MEDLINE
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