Diffusive Dynamics of Bacterial Proteome as a Proxy of Cell Death.

Autor:	Di Bari D; Università degli Studi di Perugia, Dipartimento di Fisica e Geologia, Via A. Pascoli, 06123Perugia PG, Italy.; Université Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de Physique, 38400Saint-Martin-d'Héres, France.; Institut Laue-Langevin, 38000Grenoble, France., Timr S; Laboratoire de Biochimie Théorique (UPR9080), CNRS, Université de Paris Cité, 13 Rue Pierre et Marie Curie, 75005Paris, France.; Institut de Biologie Physico-Chimique, Fondation Edmond de Rothschild, 13 Rue Pierre et Marie Curie, 75005Paris, France.; J. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, 182 23Prague 8, Czechia., Guiral M; Laboratoire de Bioénergétique et Ingénierie des Protéines, BIP, CNRS, Aix-Marseille Université, 13400Marseille, France., Giudici-Orticoni MT; Laboratoire de Bioénergétique et Ingénierie des Protéines, BIP, CNRS, Aix-Marseille Université, 13400Marseille, France., Seydel T; Institut Laue-Langevin, 38000Grenoble, France., Beck C; Institut Laue-Langevin, 38000Grenoble, France., Petrillo C; Università degli Studi di Perugia, Dipartimento di Fisica e Geologia, Via A. Pascoli, 06123Perugia PG, Italy., Derreumaux P; Laboratoire de Biochimie Théorique (UPR9080), CNRS, Université de Paris Cité, 13 Rue Pierre et Marie Curie, 75005Paris, France.; Institut de Biologie Physico-Chimique, Fondation Edmond de Rothschild, 13 Rue Pierre et Marie Curie, 75005Paris, France.; Institut Universitaire de France, 75005Paris, France., Melchionna S; ISC-CNR, Dipartimento di Fisica, Università Sapienza, 00185Rome, Italy.; Lexma Technology1337 Massachusetts Avenue, Arlington, Massachusetts02476, United States., Sterpone F; Laboratoire de Biochimie Théorique (UPR9080), CNRS, Université de Paris Cité, 13 Rue Pierre et Marie Curie, 75005Paris, France.; Institut de Biologie Physico-Chimique, Fondation Edmond de Rothschild, 13 Rue Pierre et Marie Curie, 75005Paris, France., Peters J; Université Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de Physique, 38400Saint-Martin-d'Héres, France.; Institut Laue-Langevin, 38000Grenoble, France.; Institut Universitaire de France, 75005Paris, France., Paciaroni A; Università degli Studi di Perugia, Dipartimento di Fisica e Geologia, Via A. Pascoli, 06123Perugia PG, Italy.
Jazyk:	angličtina
Zdroj:	ACS central science [ACS Cent Sci] 2023 Jan 09; Vol. 9 (1), pp. 93-102. Date of Electronic Publication: 2023 Jan 09 (Print Publication: 2023).
DOI:	10.1021/acscentsci.2c01078
Abstrakt:	Temperature variations have a big impact on bacterial metabolism and death, yet an exhaustive molecular picture of these processes is still missing. For instance, whether thermal death is determined by the deterioration of the whole or a specific part of the proteome is hotly debated. Here, by monitoring the proteome dynamics of E. coli , we clearly show that only a minor fraction of the proteome unfolds at the cell death. First, we prove that the dynamical state of the E. coli proteome is an excellent proxy for temperature-dependent bacterial metabolism and death. The proteome diffusive dynamics peaks at about the bacterial optimal growth temperature, then a dramatic dynamical slowdown is observed that starts just below the cell's death temperature. Next, we show that this slowdown is caused by the unfolding of just a small fraction of proteins that establish an entangling interprotein network, dominated by hydrophobic interactions, across the cytoplasm. Finally, the deduced progress of the proteome unfolding and its diffusive dynamics are both key to correctly reproduce the E. coli growth rate. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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