Shower water contributes viable nontuberculous mycobacteria to indoor air.

Autor:	Shen Y; Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109, USA., Haig SJ; Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109, USA., Prussin AJ 2nd; Department of Civil and Environmental Engineering, Virginia Tech, 418 Durham Hall, Blacksburg, VA 24061, USA., LiPuma JJ; Department of Pediatrics, University of Michigan Medical School, 1500 E. Medical Center Dr., Ann Arbor, MI 48109, USA., Marr LC; Department of Civil and Environmental Engineering, Virginia Tech, 418 Durham Hall, Blacksburg, VA 24061, USA., Raskin L; Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109, USA.
Jazyk:	angličtina
Zdroj:	PNAS nexus [PNAS Nexus] 2022 Nov 10; Vol. 1 (5), pp. pgac145. Date of Electronic Publication: 2022 Nov 10 (Print Publication: 2022).
DOI:	10.1093/pnasnexus/pgac145
Abstrakt:	Nontuberculous mycobacteria (NTM) are frequently present in municipal drinking water and building plumbing, and some are believed to cause respiratory tract infections through inhalation of NTM-containing aerosols generated during showering. However, the present understanding of NTM transfer from water to air is insufficient to develop NTM risk mitigation strategies. This study aimed to characterize the contribution of shower water to the abundance of viable NTM in indoor air. Shower water and indoor air samples were collected, and 16S rRNA and rpoB genes were sequenced. The sequencing results showed that running the shower impacted the bacterial community structure and NTM species composition in indoor air by transferring certain bacteria from water to air. A mass balance model combined with NTM quantification results revealed that on average 1/132 and 1/254 of NTM cells in water were transferred to air during 1 hour of showering using a rain and massage showerhead, respectively. A large fraction of the bacteria transferred from water to air were membrane-damaged, i.e. they had compromised membranes based on analysis by live/dead staining and flow cytometry. However, the damaged NTM in air were recoverable as shown by growth in a culture medium mimicking the respiratory secretions of people with cystic fibrosis, implying a potential infection risk by NTM introduced to indoor air during shower running. Among the recovered NTM, Mycobacterium mucogenicum was the dominant species as determined by rpoB gene sequencing. Overall, this study lays the groundwork for future pathogen risk management and public health protection in the built environment. (© The Author(s) 2022. Published by Oxford University Press on behalf of National Academy of Sciences.)
Databáze:	MEDLINE
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