Dietary Restriction Impacts Peripheral Circadian Clock Output Important for Longevity in Drosophila .

Autor:	Hwangbo DS; Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.; Center for Sleep & Circadian Biology, Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA.; Department of Biology, University of Louisville, Louisville, 40292, KY, USA., Kwon YJ; Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA., Iwanaszko M; Biostatistics Division, Department of Preventive Medicine, Northwestern University, Chicago, IL 60611, USA.; Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL 60208, USA.; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA., Jiang P; Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.; Center for Sleep & Circadian Biology, Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA., Abbasi L; Department of Biology, University of Louisville, Louisville, 40292, KY, USA., Wright N; Department of Biology, University of Louisville, Louisville, 40292, KY, USA., Alli S; Department of Biology, University of Louisville, Louisville, 40292, KY, USA., Hutchison AL; James Franck Institute, Department of Chemistry, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA., Dinner AR; James Franck Institute, Department of Chemistry, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA., Braun RI; Biostatistics Division, Department of Preventive Medicine, Northwestern University, Chicago, IL 60611, USA.; Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL 60208, USA.; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA., Allada R; Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.; Center for Sleep & Circadian Biology, Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jan 04. Date of Electronic Publication: 2023 Jan 04.
DOI:	10.1101/2023.01.04.522718
Abstrakt:	Circadian clocks may mediate lifespan extension by caloric or dietary restriction (DR). We find that the core clock transcription factor Clock is crucial for a robust longevity and fecundity response to DR in Drosophila . To identify clock-controlled mediators, we performed RNA-sequencing from abdominal fat bodies across the 24 h day after just 5 days under control or DR diets. In contrast to more chronic DR regimens, we did not detect significant changes in the rhythmic expression of core clock genes. Yet we discovered that DR induced de novo rhythmicity or increased expression of rhythmic clock output genes. Network analysis revealed that DR increased network connectivity in one module comprised of genes encoding proteasome subunits. Adult, fat body specific RNAi knockdown demonstrated that proteasome subunits contribute to DR-mediated lifespan extension. Thus, clock control of output links DR-mediated changes in rhythmic transcription to lifespan extension. Competing Interests: Declaration of Interests The authors declare no competing interests.
Databáze:	MEDLINE
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