A comparative analysis of microglial inducible Cre lines.

Autor: Faust TE; Department of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA., Feinberg PA; Department of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA., O'Connor C; Department of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA., Kawaguchi R; Department of Psychiatry and Neurology UCLA., Chan A; Department of Neuroscience, Icahn School of Medicine at Mount Sinai., Strasburger H; Department of Neuroscience, Icahn School of Medicine at Mount Sinai., Masuda T; Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany.; Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan., Amann L; Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany., Knobeloch KP; Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Prinz M; Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany.; Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Schaefer A; Department of Neuroscience, Icahn School of Medicine at Mount Sinai.; Max Planck Institute for Biology of Ageing, Cologne., Schafer DP; Department of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2023 Jan 09. Date of Electronic Publication: 2023 Jan 09.
DOI: 10.1101/2023.01.09.523268
Abstrakt: Cre/LoxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. The field of microglial biology has particularly benefited from this technology as microglia have historically been difficult to transduce with virus or electroporation methods for gene delivery. Here, we interrogate four of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency and spontaneous recombination, depending on the Cre line and loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency in microglia, which could be extended to other cell types. There is increasing evidence that microglia are key regulators of neural circuit structure and function. Microglia are also major drivers of a broad range of neurological diseases. Thus, reliable manipulation of their function in vivo is of utmost importance. Identifying caveats and benefits of all tools and implementing the most rigorous protocols are crucial to the growth of the field of microglial biology and the development of microglia-based therapeutics.
Databáze: MEDLINE