Microcurvature Controllable Metal-Organic Framework Nanoagents Capable of Ice-Lattice Matching for Cellular Cryopreservation.

Autor:	Jeon N; School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju61005, Republic of Korea., Jeong IH; Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul05505, Republic of Korea., Cho E; Composites Research Division, Korea Institute of Materials Science (KIMS), Changwon51508, Republic of Korea., Choi I; School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju61005, Republic of Korea., Lee J; School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju61005, Republic of Korea., Han EH; Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Cheongju28119, Republic of Korea., Lee HJ; Composites Research Division, Korea Institute of Materials Science (KIMS), Changwon51508, Republic of Korea., Lee PCW; Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul05505, Republic of Korea., Lee E; School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju61005, Republic of Korea.
Jazyk:	angličtina
Zdroj:	JACS Au [JACS Au] 2022 Dec 20; Vol. 3 (1), pp. 154-164. Date of Electronic Publication: 2022 Dec 20 (Print Publication: 2023).
DOI:	10.1021/jacsau.2c00562
Abstrakt:	Ice-binding proteins (IBPs) produced by psychrophilic organisms to adapt for the survival of psychrophiles in subzero conditions have received illustrious interest as a cryopreservation agent required for cells and tissues to completely recover after freezing/thawing. Depressing water-freezing point and avoiding ice-crystal growth affect their activities which are closely related to the presence of ice crystal well-matched binding moiety. The interaction of IBPs with ice and water is critical in enhancing their freeze avoidance against cell or tissue damage. Metal-organic frameworks (MOFs) with a controllable lattice at the molecular level and a size at the nanometer scale can offer periodically ordered ice-binding sites by modifying organic linkers and controlling microcurvature at the ice surface. Herein, zirconium (Zr)-based MOF-801 nanoparticles (NPs) with good biocompatibility were used as a cryoprotectant that is well dispersed and colloidal-stable in an aqueous solution. The MOF NP size was precisely controlled, and 10, 35, 100, and 250 nm NPs were prepared. The specific IBPs-mimicking pendants (valine and threonine) were simply introduced into the MOF NP-surface through the acrylate-based functionalization to endow with hydrophilic and hydrophobic dualities. When small-sized MOF-801 NPs were attached to ice, they confined ice growth in high curvature between the adsorption sites because of the decreased radius of the convex area of the growth region, leading to highly enhanced ice recrystallization inhibition (IRI). Surface-functionalized MOF NPs could increase the number of anchored clathrate water molecules with hydrophilic/hydrophobic balance of the ice-binding moiety, effectively inhibiting ice growth. The MOF-801 NPs were biocompatible with various cell lines regardless of concentration or NP surface-functionalization, whereas the smaller-sized surface-functionalized NPs showed a good cell recovery rate after freezing/thawing by induction of IRI. This study provides a strategy for the fabrication of low-cost, high-volume antifreeze nanoagents that can extend useful applications to organ transplantation, cord blood storage, and vaccines/drugs. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu