Hemodynamic impairment induced by Crotoxin using in vivo and ex vivo approach in a rat model.

Autor: Sartim MA; Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil; Department of Research and Development, University Nilton Lins, Manaus, Brazil; Department of Teaching and Research, Fundação de Medicina Tropical, Heitor Vieira Dourado, Manaus, Brazil., Nogueira RC; Department of Pharmacology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Cavalcante TTA; Department of Biological Sciences, Federal University of Amazonas, Manaus, Brazil., Sousa LO; Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Monteiro WM; Department of Teaching and Research, Fundação de Medicina Tropical, Heitor Vieira Dourado, Manaus, Brazil; Amazonas State University, Manaus, Brazil., Cintra ACO; Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Neto-Neves EM; Department of Physiological Sciences, Federal University of Espírito Santo, Vitoria, Brazil., Sampaio SV; Department of Clinical Analysis, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: suvilela@usp.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Mar 31; Vol. 232, pp. 123408. Date of Electronic Publication: 2023 Jan 26.
DOI: 10.1016/j.ijbiomac.2023.123408
Abstrakt: Crotalus durissus snakebite represent 10 % of snakebite cases in Brazil, which cardiovascular disorders are associated with severe cases. Considering crotoxin (CTX) as the major venom component, the present study aimed to evaluate the hemodynamic alterations induced by CTX using in vivo and ex vivo approaches in a rat model. In vivo cardiac function parameters were analyzed from anesthetized rats treated with CTX or saline only (Sham), along with serum creatine kinase MB (CK-MB) and lung myeloperoxidase. From the same animals, hearts were isolated and functional parameters evaluated in Langendorff method ex vivo. CTX binding to myoblast cell line in vitro were evaluated using confocal microscopy and flow cytometry. CTX was capable of reducing arterial and diastolic blood pressure, heart rate, along with left ventricle pressure development or decay during systole (LVdP/dt max and LVdP/dt min ) in vivo, however no differences were found in the ex vivo approach, showing that intrinsic heart function was preserved. In vitro, CTX binding to myoblast cell line was mitigated by hexamethonium, a nicotinic acetylcholine receptor antagonist. The present study has shown that CTX induce hemodynamic failure in rats, which can help improve the clinical management of cardiovascular alterations during Crotalus durissus snakebite.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Suely Vilela Sampaio reports equipment, drugs, or supplies were provided by National Council for Scientific and Technological Development. Suely Vilela Sampaio reports equipment, drugs, or supplies was provided by State of Sao Paulo Research Foundation. Marco Aurelio Sartim reports equipment, drugs, or supplies was provided by Foundation for Research Support of Amazonas State. Wuelton Marcelo Monteiro reports writing assistance was provided by Foundation for Research Support of Amazonas State.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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