Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model.

Autor: Mathur P; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France.; Institut Gustave Roussy, INSERM UMR1279, 94805, Villejuif, France., De Barros Santos C; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France., Lachuer H; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France.; Institut Gustave Roussy, INSERM UMR1279, 94805, Villejuif, France., Patat J; Institut Gustave Roussy, INSERM UMR1279, 94805, Villejuif, France.; Paris-Saclay University, 91190, Gif-sur-Yvette, France., Latgé B; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France., Radvanyi F; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France., Goud B; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France.; Institut Curie, PSL Research University, 75248, Paris, France., Schauer K; Centre National de la Recherche Scientifique, UMR144, 75005, Paris, France. kristine.schauer@gustaveroussy.fr.; Institut Curie, PSL Research University, 75248, Paris, France. kristine.schauer@gustaveroussy.fr.; Institut Gustave Roussy, INSERM UMR1279, 94805, Villejuif, France. kristine.schauer@gustaveroussy.fr.; Paris-Saclay University, 91190, Gif-sur-Yvette, France. kristine.schauer@gustaveroussy.fr.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Jan 28; Vol. 6 (1), pp. 114. Date of Electronic Publication: 2023 Jan 28.
DOI: 10.1038/s42003-023-04501-1
Abstrakt: Lysosomes orchestrate degradation and recycling of exogenous and endogenous material thus controlling cellular homeostasis. Little is known how this organelle changes during cancer. Here we investigate the intracellular landscape of lysosomes in a cellular model of bladder cancer. Employing standardized cell culture on micropatterns we identify a phenotype of peripheral lysosome positioning prevailing in bladder cancer cell lines but not normal urothelium. We show that lysosome positioning is controlled by phosphatidylinositol-3-phosphate (PtdIns3P) levels on endomembranes which recruit FYVE-domain containing proteins for lysosomal dispersion. We identify transcription factor EB (TFEB) as an upstream regulator of PtdIns3P production by VPS34 that is activated in aggressive bladder cancer cells with peripheral lysosomes. This conceptually clarifies the dual role of TFEB as regulator of endosomal maturation and autophagy, two distinct processes controlled by PtdIns3P. Altogether, our findings uncover peripheral lysosome positioning, resulting from PtdIns3P production downstream of TFEB activation, as a potential biomarker for bladder cancer.
(© 2023. The Author(s).)
Databáze: MEDLINE
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