A candidate transporter allowing symbiotic dinoflagellates to feed their coral hosts.

Autor:	Maor-Landaw K; School of Biosciences, The University of Melbourne, Parkville, VIC, Australia.; Department of Marine Biology, The Leon H. Charney School of Marine Sciences, University of Haifa, Haifa, Israel., Eisenhut M; Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Universitätsstraße 1, D-40225, Düsseldorf, Germany.; Computational Biology, Faculty of Biology, CeBiTec, Bielefeld University, Bielefeld, Germany., Tortorelli G; School of Biosciences, The University of Melbourne, Parkville, VIC, Australia.; Coral Resilience Lab, Hawai'i Institute of Marine Biology, Kāne'ohe, HI, USA., van de Meene A; School of Biosciences, The University of Melbourne, Parkville, VIC, Australia., Kurz S; Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Universitätsstraße 1, D-40225, Düsseldorf, Germany., Segal G; Biological Optical Microscopy Platform, The University of Melbourne, Parkville, VIC, Australia., van Oppen MJH; School of Biosciences, The University of Melbourne, Parkville, VIC, Australia.; Australian Institute of Marine Science, Townsville, QLD, Australia., Weber APM; Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Universitätsstraße 1, D-40225, Düsseldorf, Germany., McFadden GI; School of Biosciences, The University of Melbourne, Parkville, VIC, Australia. gim@unimelb.edu.au.
Jazyk:	angličtina
Zdroj:	ISME communications [ISME Commun] 2023 Jan 28; Vol. 3 (1), pp. 7. Date of Electronic Publication: 2023 Jan 28.
DOI:	10.1038/s43705-023-00218-8
Abstrakt:	The symbiotic partnership between corals and dinoflagellate algae is crucial to coral reefs. Corals provide their algal symbionts with shelter, carbon dioxide and nitrogen. In exchange, the symbiotic algae supply their animal hosts with fixed carbon in the form of glucose. But how glucose is transferred from the algal symbiont to the animal host is unknown. We reasoned that a transporter resident in the dinoflagellate cell membrane would facilitate outward transfer of glucose to the surrounding host animal tissue. We identified a candidate transporter in the cnidarian symbiont dinoflagellate Breviolum minutum that belongs to the ubiquitous family of facilitative sugar uniporters known as SWEETs (sugars will eventually be exported transporters). Previous gene expression analyses had shown that BmSWEET1 is upregulated when the algae are living symbiotically in a cnidarian host by comparison to the free-living state [1, 2]. We used immunofluorescence microscopy to localise BmSWEET1 in the dinoflagellate cell membrane. Substrate preference assays in a yeast surrogate transport system showed that BmSWEET1 transports glucose. Quantitative microscopy showed that symbiotic B. minutum cells have significantly more BmSWEET1 protein than free-living cells of the same strain, consistent with export during symbiosis but not during the free-living, planktonic phase. Thus, BmSWEET1 is in the right place, at the right time, and has the right substrate to be the transporter with which symbiotic dinoflagellate algae feed their animal hosts to power coral reefs. (© 2023. The Author(s).)
Databáze:	MEDLINE
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