| Autor: |
Ahn SY; Department of Medicine, Yonsei University College of Medicine, Republic of Korea., Jung EH; Department of Medicine, Yonsei University College of Medicine, Republic of Korea., Ahn H; Department of Medicine, Yonsei University College of Medicine, Republic of Korea.; Department of Ophthalmology, The Institute of Vision Research, Yonsei University College of Medicine, Republic of Korea., Lee JS; Department of Medicine, Yonsei University College of Medicine, Republic of Korea.; Department of Ophthalmology, The Institute of Vision Research, Yonsei University College of Medicine, Republic of Korea., Bak JH; Department of Mechanical Engineering, Hanyang University, Republic of Korea., Kim ED; Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA., Song JH; Korea Mouse Sensory Phenotyping Center (KMSPC), Yonsei University College of Medicine, Republic of Korea., Shin HS; Department of Ophthalmology, The Institute of Vision Research, Yonsei University College of Medicine, Republic of Korea.; Korea Mouse Sensory Phenotyping Center (KMSPC), Yonsei University College of Medicine, Republic of Korea., Jamiyansharav M; Department of Medicine, Yonsei University College of Medicine, Republic of Korea., Seo KY; Department of Medicine, Yonsei University College of Medicine, Republic of Korea.; Department of Ophthalmology, The Institute of Vision Research, Yonsei University College of Medicine, Republic of Korea. |
| Abstrakt: |
In the field of visual science study using rodents, several assessment methods have been developed for measuring visual function. However, methods such as electroretinograms tests, visual evoked potentials tests and maze tests have limitations in that they measure function of only a specific type of cells, are difficult to quantify or require sufficient training time. The method which uses an optokinetic reflex and optomotor response, a compensatory eye and head movement in response to changes in the visual scene, became the most widely used method. However, this method requires highly trained experimenters and is time consuming. We showed that measured visual acuity values are significantly different between beginner and expert. Here we suggest an automated optometry program, 'SKY optomotry', which automatically tracks rodents' optomotor response to overcome subjectivity and the lengthy scoring procedure of the existing method. To evaluate the performance of SKY optomotry using 8-12-week-old C57BL/6 mice we compared the binomial decision of SKY optomotry with a skilled expert, and the area under the curve of SKY optomotry was 0.845. Comparing the final visual acuity, the intraclass correlation coefficient value between SKY optomotry and an expert was 0.860 (95% confidence interval (CI) 0.709-0.928), whereas that between an expert and a beginner was 0.642 (95% CI 0.292-0.811). SKY optomotry showed an excellent level of performance with good inter-rater agreements based on the visual acuity measured by an expert. With the use of our application, researchers will be able to test an experimental animal's eyesight more accurately while saving time on specialized training. |
