| Autor: |
Pappalardo A; Department of Dermatology, Columbia University Irving Medical Center, New York, NY 10032, USA., Alvarez Cespedes D; Department of Dermatology, Columbia University Irving Medical Center, New York, NY 10032, USA., Fang S; Department of Mechanical Engineering, School of Engineering and Applied Science, Columbia University, New York, NY 10027, USA., Herschman AR; Department of Mechanical Engineering, School of Engineering and Applied Science, Columbia University, New York, NY 10027, USA., Jeon EY; Department of Dermatology, Columbia University Irving Medical Center, New York, NY 10032, USA., Myers KM; Department of Mechanical Engineering, School of Engineering and Applied Science, Columbia University, New York, NY 10027, USA., Kysar JW; Department of Mechanical Engineering, School of Engineering and Applied Science, Columbia University, New York, NY 10027, USA.; Department of Otolaryngology - Head & Neck Surgery, Columbia University Irving Medical Center, New York, NY 10032, USA., Abaci HE; Department of Dermatology, Columbia University Irving Medical Center, New York, NY 10032, USA. |
| Abstrakt: |
Despite the advancements in skin bioengineering, 3D skin constructs are still produced as flat tissues with open edges, disregarding the fully enclosed geometry of human skin. Therefore, they do not effectively cover anatomically complex body sites, e.g., hands. Here, we challenge the prevailing paradigm by engineering the skin as a fully enclosed 3D tissue that can be shaped after a body part and seamlessly transplanted as a biological clothing. Our wearable edgeless skin constructs (WESCs) show enhanced dermal extracellular matrix (ECM) deposition and mechanical properties compared to conventional constructs. WESCs display region-specific cell/ECM alignment, as well as physiologic anisotropic mechanical properties. WESCs replace the skin in full-thickness wounds of challenging body sites (e.g., mouse hindlimbs) with minimal suturing and shorter surgery time. This study provides a compelling technology that may substantially improve wound care and suggests that the recapitulation of the tissue macroanatomy can lead to enhanced biological function. |
