In vitro leishmanicidal effect of Yangambin and Epi-yangambin lignans isolated from Ocotea fasciculata (Nees) Mez.

Autor: Rebouças-Silva J; Laboratory of Inflammation and Biomarkers, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.; Faculty of Medicine of Bahia, Federal University of Bahia (UFBA), Salvador, Bahia, Brazil., Santos GF; Laboratory of Inflammation and Biomarkers, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil., Filho JMB; Post-Graduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa, Paraíba, Brazil., Berretta AA; Laboratory of Research, Development and Innovation, Apis Flora Industrial e Comercial Ltda, Ribeirão Preto, São Paulo, Brazil., Marquele-Oliveira F; Laboratory of Research, Development and Innovation, Eleve Science Research and Development, Ribeirão Preto, São Paulo, Brazil., Borges VM; Laboratory of Inflammation and Biomarkers, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.; Faculty of Medicine of Bahia, Federal University of Bahia (UFBA), Salvador, Bahia, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2023 Jan 10; Vol. 12, pp. 1045732. Date of Electronic Publication: 2023 Jan 10 (Print Publication: 2022).
DOI: 10.3389/fcimb.2022.1045732
Abstrakt: Introduction: Yangambin and epi-yangambin are the main lignans found in Louro-de-Cheiro [ Ocotea fasciculata (Nees) Mez], a tree native to the Atlantic forests of northeastern Brazil whose leaves and bark are widely used in folk medicine. The present study investigated the leishmanicidal and immunomodulatory effects of both lignans in in vitro models of infection by Leishmania amazonensis or Leishmania braziliensis , both etiological agents of Cutaneous Leishmaniasis in Brazil.
Methods: Bone marrow-derived mouse macrophages were infected with L. amazonensis or L. braziliensis and then treated for 48 h at varying concentrations of yangambin or epi-yangambin.
Results: Yangambin and epi-yangambin were found to reduce the intracellular viability of either Leishmania species in a concentration-dependent manner, with respective IC 50 values of: 43.9 ± 5 and 22.6 ± 4.9 µM for L. amazonensis , compared to IC 50 values of 76 ± 17 and 74.4 ± 9.8 µM for L. braziliensis . In this context, epi-yangambin proved more selective and effective against in vitro infection by L. amazonensis . However, both lignans were found to distinctly modulate the production of inflammatory mediators and other cytokines by macrophages infected by either of the Leishmania species evaluated. While yangambin increased the production of IL-10 by L. braziliensis- infected macrophages, both compounds were observed to lower the production of NO, PGE 2 , IL-6 and TNF-α in both Leishmania species.
Discussion: The present results serve to encourage the development of novel studies aimed at screening natural bioactive compounds with the hope of discovering new therapeutic options for the treatment of Cutaneous Leishmaniasis.
Competing Interests: Author AB was employed by Apis Flora Industrial e Comercial Ltda. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Rebouças-Silva, Santos, Filho, Berretta, Marquele-Oliveira and Borges.)
Databáze: MEDLINE
Externí odkaz: