Quantitative Assessment of Dentinal Tubule Disinfection in Absence of Biofilm on Root Canal Walls: An in vitro Study.

Autor: Byakova SF; Department of Therapeutic Dentistry, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian Federation, Mojaiskii val str, Moscow, Russia., Dezhurko-Korol VA; Department of Therapeutic Dentistry, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian Federation, Mojaiskii val str, Moscow, Russia., Novozhilova NE; Department of Therapeutic Dentistry, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian Federation, Mojaiskii val str, Moscow, Russia., Makeeva IM; Department of Therapeutic Dentistry, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of the Russian Federation, Mojaiskii val str, Moscow, Russia., Lukashev AN; E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), M. Pirogovskaya str., Moscow, Russia., Akhmadishina LV; E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), M. Pirogovskaya str., Moscow, Russia., Semenov AM; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia., Moisenovich MM; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia., Arkhipova AY; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia., Ponirovsky EN; E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), M. Pirogovskaya str., Moscow, Russia.
Jazyk: angličtina
Zdroj: Iranian endodontic journal [Iran Endod J] 2020 Summer; Vol. 15 (3), pp. 155-165.
DOI: 10.22037/iej.v15i3.27453
Abstrakt: Introduction: This study aimed at assessing the quantitative effect of calcium hydroxide, 2% chlorhexidine gel, and 1.5% chlorhexidine linked to xanthan gel specifically against intratubular bacteria.
Methods and Materials: Fifty-two semi-cylindrical bovine dentin specimens were infected with Enterococcus (E.) faecalis by centrifugation with subsequent 7-days incubation. The surface of specimens was disinfected with 3% H 2 O 2 . Scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and the count of bacterial colony-forming units (CFU/mg) were used to assess dentin infection. A total of 40 specimens were incubated for 2 weeks with one of the intracanal medication applied (10 samples for each group): 1) calcium hydroxide, 2) 2% chlorhexidine gel, 3) 1.5% chlorhexidine linked to xanthan gel and 4) sterile saline. Final passive ultrasonic irrigation with 3% sodium hypochlorite was performed in half of the total specimens. The effect of intracanal medications and irrigation against intratubular bacteria was assessed by bacterial culturing of dentin shavings. Two-Way ANOVA model was applied followed by post-hoc Tukey's test for multiple pair-wise comparisons of mean CFU/mg values.
Results: SEM, CLSM, and bacterial culturing confirmed the absence of the surface biofilm on the root canal wall and showed vital intratubular bacteria at the depth up to 700  m. Two-week application of 1.5% chlorhexidine with xanthan gel and 2% chlorhexidine gel significantly decreased intratubular bacterial counts compared with saline ( P =0.0003 and P =0.0005, respectively). Subsequent passive ultrasonic irrigation with 3% sodium hypochlorite significantly reduced the number of intratubular bacteria in all groups except for the group with 1.5% chlorhexidine-xanthan gel ( P =0.0054).
Conclusion: This modified ex vivo model study showed ultrasonically activated irrigation with sodium hypochlorite had greater effect on intratubular bacteria counts compared with 2-week application of intracanal medications.
Competing Interests: ‘None declared’.
Databáze: MEDLINE
Externí odkaz: