Effect of cigarette smoke on mucosal vaccine response with activation of plasmacytoid dendritic cells: The outcomes of in vivo and in vitro experiments.

Autor: Suzuki F; Department of Forensic Medicine and Human Genetics, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: fumikoszk1017@yahoo.co.jp., Maeyama JI; Reseach Center for Biological Products in the Next Generation, National Institute of Infectious Diseases, Musashimurayama-shi, Tokyo 208-0011, Japan. Electronic address: meymj@nih.go.jp., Kubota A; Laboratory of Host Defense, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: tanda@arrow.ocn.ne.jp., Nishimune A; Laboratory of Host Defense, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: rsj44017@nifty.com., Horiguchi S; Department of Anatomy and Neuroscience, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: jurlique0316@yahoo.co.jp., Takii T; Department of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya 467-8603, Japan; Department of Mycobacterium Reference and Research, the Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose, Tokyo 204-8533, Japan. Electronic address: t-takii@jata.or.jp., Urasaki Y; Health Administration Center, University of Fukui, 3-9-1, Bunkyo, Fukui-shi, Fukui 910-8507, Japan. Electronic address: urasakiy@u-fukui.ac.jp., Shimada I; Department of Forensic Medicine and Human Genetics, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan. Electronic address: ichi6shimada@gmail.com., Iho S; Laboratory of Host Defense, School of Medical Sciences, University of Fukui, 23-3 Matsuoka shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan; IFN & Host-defence Research Laboratory, Division of Basic Research, Luis Pasteur Center for Medical Research, 103-5, Tanaka, Monzen-cho, Sakyo-ku, Kyoto, 606-8225, Japan. Electronic address: tr378838@wb3.so-net.ne.jp.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2023 Feb 17; Vol. 41 (8), pp. 1447-1456. Date of Electronic Publication: 2023 Jan 24.
DOI: 10.1016/j.vaccine.2023.01.019
Abstrakt: Mucosal vaccines offer several advantages over transdermal vaccines, including the ability to acquire systemic and mucosal immunities. Smoking is a huge public health threat and major risk factor for various diseases that exacerbate or prolong respiratory symptoms and conditions. However, its impact on the efficacy of mucosal vaccines remains partially explored. Thus, this study investigates the effects of smoking on mucosal vaccine reactivity by assessing the induction of Th1 immunity, a vital response in infection defense. Cigarette smoke condensate was prepared as a substitute for mainstream smoke. We intranasally administered diphtheria toxoid as an antigen and natural CpG oligonucleotide G9.1, which enhances the Th1-type antibody (Ab) response in a plasmacytoid dendritic cells (pDCs) dependent manner, as an adjuvant to mice to assess the effect of cigarette smoke condensate on Ab responses. The mechanism of its effect was evaluated using human peripheral blood mononuclear cells and their pDC-rich fraction cultured with or without G9.1. In mice, cigarette smoke condensate tended to decrease diphtheria toxoid-specific Ab response, with a higher reduction in Th1-type IgG2 Ab response than in Th2-type IgG1 Ab response. In human peripheral blood mononuclear cells, cigarette smoke condensate significantly reduced the induction of IFN-α production by G9.1. Moreover, G9.1-induced increases in the CD83 expression in pDCs and the CD80 expression in DCs were suppressed via treatment with cigarette smoke condensate. Among the mechanisms suggested were decreased expression of toll-like receptor 9 mRNA, decreased expression of mRNA for IFN regulatory factor 7, and increased CpG methylation of its promoter region. The analysis of Tbet and GATA3 expressions revealed that cigarette smoke condensate exhibits Th1-directed immunostimulatory activity at a steady state but becomes more Th2-directed under G9.1 stimulation. In conclusion, smoking could reduce mucosal vaccine responses by decreasing pDC activation and, consequently, Th1-dominant immunity.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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