Cancer cells inhibition by cationic carbon dots targeting the cellular nucleus.

Autor: Chen J; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Li F; Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, FL 33136, USA., Gu J; Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, FL 33136, USA., Zhang X; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Bartoli M; Department of Applied Science and Technology, Politecnico di Torino, Italy., Domena JB; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Zhou Y; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA; C-Dots, LLC, Miami, FL 33136, USA., Zhang W; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Paulino V; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., C L B Ferreira B; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Michael Brejcha N; Department of Biochemistry and Molecular Biology, University of Miami, Coral Gables, FL 33146, USA., Luo L; Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, FL 33136, USA., Arduino C; Department of Applied Science and Technology, Politecnico di Torino, Italy., Verde F; Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, FL 33136, USA., Zhang F; Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, FL 33136, USA., Zhang F; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Tagliaferro A; Department of Applied Science and Technology, Politecnico di Torino, Italy., Olivier JH; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA., Zhang Y; Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, FL 33136, USA. Electronic address: yzhang4@med.miami.edu., Leblanc RM; Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA. Electronic address: rml@miami.edu.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2023 May; Vol. 637, pp. 193-206. Date of Electronic Publication: 2023 Jan 21.
DOI: 10.1016/j.jcis.2023.01.086
Abstrakt: Nucleus targeting is tremendously important in cancer therapy. Cationic carbon dots (CCDs) are potential nanoparticles which might enter cells and penetrate nuclear membranes. Although some CCDs have been investigated in nucleus targeting and applied in nuclear imaging, the CCDs derived from drugs, that are able to target the nucleus, bind with DNA and inhibit the growth of cancer cells have not been reported. In this project, 1, 2, 4, 5-benzenetetramine (Y15, a focal adhesion kinase inhibitor) derived cationic carbon dots (Y15-CDs) were prepared via a hydrothermal approach utilizing Y15, folic acid and 1,2-ethylenediamine as precursors. Based on the structural, optical, and morphologic characterizations, Y15-CDs possess rich amine groups and nitrogen in structure, an excitation-dependent photoluminescence emission, and a small particle size of 2 to 4 nm. The DNA binding experiments conducted through agarose gel electrophoresis, UV-vis absorption, fluorescence emission, and circular dichroism spectroscopies, prove that Y15-CDs might bind with DNA via electrostatic interactions and partially intercalative binding modes. In addition, the cell imaging and cytotoxicity studies in human foreskin fibroblasts (HFF), prostate cancer (PC3) and osteosarcoma cells (U2OS) indicate the nucleus targeting and anticancer abilities of Y15-CDs. Most interestingly, Y15-CDs exhibit a higher cytotoxicity to cancer cells (PC3 and U2OS) than to normal cells (HFF), inferring that Y15-CDs might be potentially applied in cancer therapy.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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